TY - JOUR
T1 - Effect of ditaxin and heteranthin and inhibitory effect of ditaxis heterantha extract on l5178Y Tumor development in mice
AU - Jaramillo-Flores, María Eugenia
AU - Permady, Herry Heriyati
AU - Puebla-Pérez, Ana Maria
AU - Padilla, Eduardo
AU - Lugo-Cervantes, Eugenia Del Carmen
AU - Delgado-Saucedo, Jorge Ivan
AU - Ramón-Gallegos, Eva
PY - 2011/5/1
Y1 - 2011/5/1
N2 - Ditaxis heterantha seeds are used as spices for flavoring and coloring food. Two new apocarotenoids derived from the seeds, heteranthin and ditaxin, were evaluated for their in vitro cytotoxic effects in murine lymphoma cells lines. Bioabsorption in mice and preventive and antitumor effects of the apocarotenoids were determined. Ditaxin and heteranthin showed cytotoxic effects in vitro against murine malignant cells and normal splenocyte cells. The 50% inhibitory concentration (IC50) for ditaxin in splenocytes was 0.1825 mM; in L5178Y, the IC50 was 0.1923 mM. The heteranthin IC 50 in splenocytes was 0.1325 mM; in L5178Y, the value was 0.3889 mM. The maximum ditaxin plasma concentration was found after 2 hours of administration (mean±standard deviation, 7.5±2.05 μg/mL). Oral administration of the D. heterantha extract (100 mg/kg per day) for 14 days after the L5178Y lymphoma cell implantation showed no significant effect compared with groups that were not pretreated. However, tumor inhibition in groups treated intraperitoneally before inoculation with the L5178Y cells showed a significant difference (P<.001) compared with the groups not pretreated.
AB - Ditaxis heterantha seeds are used as spices for flavoring and coloring food. Two new apocarotenoids derived from the seeds, heteranthin and ditaxin, were evaluated for their in vitro cytotoxic effects in murine lymphoma cells lines. Bioabsorption in mice and preventive and antitumor effects of the apocarotenoids were determined. Ditaxin and heteranthin showed cytotoxic effects in vitro against murine malignant cells and normal splenocyte cells. The 50% inhibitory concentration (IC50) for ditaxin in splenocytes was 0.1825 mM; in L5178Y, the IC50 was 0.1923 mM. The heteranthin IC 50 in splenocytes was 0.1325 mM; in L5178Y, the value was 0.3889 mM. The maximum ditaxin plasma concentration was found after 2 hours of administration (mean±standard deviation, 7.5±2.05 μg/mL). Oral administration of the D. heterantha extract (100 mg/kg per day) for 14 days after the L5178Y lymphoma cell implantation showed no significant effect compared with groups that were not pretreated. However, tumor inhibition in groups treated intraperitoneally before inoculation with the L5178Y cells showed a significant difference (P<.001) compared with the groups not pretreated.
KW - Ditaxis heterantha
KW - anti-tumor
KW - apocarotenoids
KW - cytotoxic
KW - ditaxin
KW - heteranthin
UR - http://www.scopus.com/inward/record.url?scp=79956079746&partnerID=8YFLogxK
U2 - 10.1089/jmf.2009.0103
DO - 10.1089/jmf.2009.0103
M3 - Artículo
C2 - 21480797
SN - 1096-620X
VL - 14
SP - 462
EP - 467
JO - Journal of Medicinal Food
JF - Journal of Medicinal Food
IS - 5
ER -