TY - JOUR
T1 - Effect of barium addition on hydrolytic enzymatic activities in food waste degradation under anaerobic conditions
AU - Ale, Fuad
AU - Castro-Muñoz, Roberto
AU - Barragán-Huerta, Blanca Estela
AU - Rodríguez-Nava, Odín
N1 - Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/11
Y1 - 2020/11
N2 - Enzymatic hydrolysis of complex components of residual materials, such as food waste, is a rate-limiting step that conditionates the production rate of biofuels. Research into the anaerobic degradation of cellulose and starch, which are abundant components in organic waste, could contribute to optimize biofuels production processes. In this work, a lab-scale anaerobic semi-continuous hydrolytic reactor was operated for 171 days using food waste as feedstock; the effect of Ba2+ dosage over the activity of five hydrolytic enzymes was also evaluated. No significant effects were observed on the global performance of the hydrolytic process during the steady-state of the operation of the reactor, nevertheless, it was detected that Ba2+ promoted β-amylases activity by 76%, inhibited endoglucanases and α-amylases activity by 39 and 20%, respectively, and had no effect on β-glucosidases and glucoamylases activity. The mechanisms that rule the observed enzymatic activity changes remain unknown; however, the discussion in this paper provides hypothetical explanations for further research.
AB - Enzymatic hydrolysis of complex components of residual materials, such as food waste, is a rate-limiting step that conditionates the production rate of biofuels. Research into the anaerobic degradation of cellulose and starch, which are abundant components in organic waste, could contribute to optimize biofuels production processes. In this work, a lab-scale anaerobic semi-continuous hydrolytic reactor was operated for 171 days using food waste as feedstock; the effect of Ba2+ dosage over the activity of five hydrolytic enzymes was also evaluated. No significant effects were observed on the global performance of the hydrolytic process during the steady-state of the operation of the reactor, nevertheless, it was detected that Ba2+ promoted β-amylases activity by 76%, inhibited endoglucanases and α-amylases activity by 39 and 20%, respectively, and had no effect on β-glucosidases and glucoamylases activity. The mechanisms that rule the observed enzymatic activity changes remain unknown; however, the discussion in this paper provides hypothetical explanations for further research.
KW - Amylases
KW - Anaerobic digestion
KW - Cellulases
KW - Enzymatic hydrolysis
KW - Food waste
UR - http://www.scopus.com/inward/record.url?scp=85094666690&partnerID=8YFLogxK
U2 - 10.3390/pr8111371
DO - 10.3390/pr8111371
M3 - Artículo
AN - SCOPUS:85094666690
SN - 2227-9717
VL - 8
SP - 1
EP - 16
JO - Processes
JF - Processes
IS - 11
M1 - 1371
ER -