TY - JOUR
T1 - Dna barcode analysis of the endangered green turtle (Chelonia mydas) in mexico
AU - Camacho-Sánchez, Fátima Yedith
AU - Aguirre, A. Alonso
AU - Narváez-Zapata, José Alberto
AU - Zavala-Norzagaray, Alan A.
AU - Ley-Quiñónez, Cesar P.
AU - Acosta-Sánchez, H. Hugo
AU - Rodriguez-González, Hervey
AU - Delgado-Trejo, Carlos
AU - Reyes-López, Miguel Angel
N1 - Publisher Copyright:
© 2021, Canadian Science Publishing. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Technological and analytical advances to study evolutionary biology, ecology, and conservation of green turtles (Chelonia mydas) are realized through molecular approaches including DNA barcoding. We characterized the usefulness of COI DNA barcodes in green turtles in Mexico to better understand genetic divergence and other genetic parameters of this species. We analyzed 63 sequences, including 25 from green turtle field specimens collected from the Gulf of Mexico and from the Mexican Pacific and 38 already present in the Barcode of Life Data Systems (BOLD). A total of 13 haplotypes were identified with four novel haplotypes from the Pacific Ocean and three novel haplotypes from the Atlantic Ocean. Intraspecific distance values among COI gene sequences by two different models were 0.01, demonstrating that there is not a subdivision for green turtle species. Otherwise, the interspecific distance interval ranged from 0.07 to 0.13, supporting a clear subdivision among all sea turtle species. Haplotype and total nucleotide diversity values of the COI gene reflect a medium genetic diversity average. Green turtles of the Mexican Pacific showed common haplotypes to some Australian and Chinese turtles, but different from the haplotypes of the Mexican Atlantic. COI analysis revealed new haplotypes and confirmed that DNA barcodes were useful for evaluation of the population diversity of green turtles in Mexico.
AB - Technological and analytical advances to study evolutionary biology, ecology, and conservation of green turtles (Chelonia mydas) are realized through molecular approaches including DNA barcoding. We characterized the usefulness of COI DNA barcodes in green turtles in Mexico to better understand genetic divergence and other genetic parameters of this species. We analyzed 63 sequences, including 25 from green turtle field specimens collected from the Gulf of Mexico and from the Mexican Pacific and 38 already present in the Barcode of Life Data Systems (BOLD). A total of 13 haplotypes were identified with four novel haplotypes from the Pacific Ocean and three novel haplotypes from the Atlantic Ocean. Intraspecific distance values among COI gene sequences by two different models were 0.01, demonstrating that there is not a subdivision for green turtle species. Otherwise, the interspecific distance interval ranged from 0.07 to 0.13, supporting a clear subdivision among all sea turtle species. Haplotype and total nucleotide diversity values of the COI gene reflect a medium genetic diversity average. Green turtles of the Mexican Pacific showed common haplotypes to some Australian and Chinese turtles, but different from the haplotypes of the Mexican Atlantic. COI analysis revealed new haplotypes and confirmed that DNA barcodes were useful for evaluation of the population diversity of green turtles in Mexico.
KW - COI gene
KW - Chelonia mydas
KW - DNA barcodes
KW - Green turtle
KW - Mexico
UR - http://www.scopus.com/inward/record.url?scp=85114339728&partnerID=8YFLogxK
U2 - 10.1139/gen-2019-0213
DO - 10.1139/gen-2019-0213
M3 - Artículo
C2 - 33555972
AN - SCOPUS:85114339728
SN - 0831-2796
VL - 64
SP - 879
EP - 891
JO - Genome
JF - Genome
IS - 9
ER -