TY - JOUR
T1 - Distribution of dystrophin- and utrophin-associated protein complexes during activation of human neutrophils
AU - Cerecedo, Doris
AU - Cisneros, Bulmaro
AU - Gómez, Pablo
AU - Galván, Iván J.
N1 - Funding Information:
We thank Dr. Mornet for the generous gift of H4 and K7 antibodies. This work was supported by research grants Consejo Nacional para la Ciencia y la Tecnología (CONACyT) (México, D.F.) CB2007-79700 and Secretaría de Investigación y Posgrado (SIP) (México, D.F.) SIP0298 to D.C.
PY - 2010/8
Y1 - 2010/8
N2 - Objective: Dystrophins, utrophins, and their associated proteins are involved in structural and signaling roles in nonmuscle tissues; however, description of these proteins in neutrophils remained unexplored. Therefore we characterize the pattern expression, and the cellular distribution of dystrophin and utrophin gene products and dystrophin-associated proteins (i.e., β-dystroglycan, α-syntrophin, and α-dystrobrevins) in relation to actin filaments in resting and activated with formyl-methionyl-leucyl-phenylalanine human neutrophils. Materials and Methods: Resting and fMLP-activated human neutrophils were analyzed by immunoblot and by confocal microscopy analysis. Immunoprecipitation assays were performed to corroborate the presence of protein complexes. Results: Immunoprecipitation assays and confocal analysis demonstrated the presence of two dystrophin-associated protein complexes in resting and activated neutrophils: the former formed by Dp71d/Dp71Δ110 m and dystrophin-associated proteins (β-dystroglycan, α-syntrophin, α-dystrobrevin-1, and -2), while the latter contains Up400, instead of Dp71d/Dp71Δ110 m, as a central component of the dystrophin-associated protein complexes (DAPC). Confocal analysis also showed the subcellular redistribution of Dp71d/Dp71Δ110 m∼DAPC and Up400∼DAPC in F-actin-based structures displayed during activation process with fMLP. Conclusions: Our study showed the existence of two protein complexes formed by Dp71d/Dp71Δ110 m or Up400 associated with DAPs in resting and fMLP-treated human polymorphonuclears. The interaction of these complexes with the actin cytoskeleton is indicative of their dynamic participation in the chemotaxis process.
AB - Objective: Dystrophins, utrophins, and their associated proteins are involved in structural and signaling roles in nonmuscle tissues; however, description of these proteins in neutrophils remained unexplored. Therefore we characterize the pattern expression, and the cellular distribution of dystrophin and utrophin gene products and dystrophin-associated proteins (i.e., β-dystroglycan, α-syntrophin, and α-dystrobrevins) in relation to actin filaments in resting and activated with formyl-methionyl-leucyl-phenylalanine human neutrophils. Materials and Methods: Resting and fMLP-activated human neutrophils were analyzed by immunoblot and by confocal microscopy analysis. Immunoprecipitation assays were performed to corroborate the presence of protein complexes. Results: Immunoprecipitation assays and confocal analysis demonstrated the presence of two dystrophin-associated protein complexes in resting and activated neutrophils: the former formed by Dp71d/Dp71Δ110 m and dystrophin-associated proteins (β-dystroglycan, α-syntrophin, α-dystrobrevin-1, and -2), while the latter contains Up400, instead of Dp71d/Dp71Δ110 m, as a central component of the dystrophin-associated protein complexes (DAPC). Confocal analysis also showed the subcellular redistribution of Dp71d/Dp71Δ110 m∼DAPC and Up400∼DAPC in F-actin-based structures displayed during activation process with fMLP. Conclusions: Our study showed the existence of two protein complexes formed by Dp71d/Dp71Δ110 m or Up400 associated with DAPs in resting and fMLP-treated human polymorphonuclears. The interaction of these complexes with the actin cytoskeleton is indicative of their dynamic participation in the chemotaxis process.
UR - http://www.scopus.com/inward/record.url?scp=77954480344&partnerID=8YFLogxK
U2 - 10.1016/j.exphem.2010.04.010
DO - 10.1016/j.exphem.2010.04.010
M3 - Artículo
SN - 0301-472X
VL - 38
SP - 618-628.e3
JO - Experimental Hematology
JF - Experimental Hematology
IS - 8
ER -