TY - JOUR
T1 - Differentially regulated genes in Solanum tuberosum in response to " Mexican potato purple top phytoplasma" infection
AU - Longoria-Espinoza, Rosa María
AU - Douriet-Gámez, Nadia Rubí
AU - López-Meyer, Melina
AU - Quiroz-Figueroa, Francisco
AU - Bueno-Ibarra, Mario
AU - Méndez-Lozano, Jesús
AU - Santos-Cervantes, María Elena
AU - Félix-Gastélum, Rubén
AU - Chávez-Medina, Jesús Alicia
AU - Leyva-López, Norma Elena
N1 - Funding Information:
We thank the Consejo Nacional de Ciencia y Tecnología (Project SEP-CONACYT-56168 ), the Consejo Estatal de Ciencia y Tecnología del Estado de Sinaloa , and the Instituto Politécnico Nacional for their economic support of this research project. We also thank Ana Lucia Alvarado-García for editing the figures.
PY - 2013/1
Y1 - 2013/1
N2 - Potato plants infected by phytoplasmas exhibit an array of symptoms that suggest interference within the normal balance of plant metabolism. Suppression subtractive hybridization (SSH), macroarray screening and real-time PCR were performed to identify differentially expressed genes in phytoplasma-infected in vitro potato plants. Gene expression analysis revealed the up-regulation of genes involved in several metabolic pathways, including ERF, WRKY, HSP90, SAP6, AN1-like ZFP, and an unknown protein; the down-regulation of genes encoding monodehydroascorbate reductase and a putative membrane protein was also observed. The results presented here provide the groundwork for addressing new questions to better understand the phytoplasma-plant interaction.
AB - Potato plants infected by phytoplasmas exhibit an array of symptoms that suggest interference within the normal balance of plant metabolism. Suppression subtractive hybridization (SSH), macroarray screening and real-time PCR were performed to identify differentially expressed genes in phytoplasma-infected in vitro potato plants. Gene expression analysis revealed the up-regulation of genes involved in several metabolic pathways, including ERF, WRKY, HSP90, SAP6, AN1-like ZFP, and an unknown protein; the down-regulation of genes encoding monodehydroascorbate reductase and a putative membrane protein was also observed. The results presented here provide the groundwork for addressing new questions to better understand the phytoplasma-plant interaction.
KW - Differentially expressed genes
KW - Macroarray screening
KW - PPT disease
KW - Phytoplasma-plant interaction
KW - Real-time PCR
KW - SSH
KW - Solanum tuberosum L.
UR - http://www.scopus.com/inward/record.url?scp=84870703129&partnerID=8YFLogxK
U2 - 10.1016/j.pmpp.2012.10.001
DO - 10.1016/j.pmpp.2012.10.001
M3 - Artículo
AN - SCOPUS:84870703129
SN - 0885-5765
VL - 81
SP - 33
EP - 44
JO - Physiological and Molecular Plant Pathology
JF - Physiological and Molecular Plant Pathology
ER -