TY - JOUR
T1 - Differential expression of surface glycoconjugates on Entamoeba histolytica and Entamoeba dispar
AU - Pacheco-Yépez, Judith
AU - Campos-Rodríguez, Rafael
AU - Rojas-Hernández, Saúl
AU - Serrano-Luna, José J.
AU - Rivera-Aguilar, Víctor
AU - Villa-Treviño, Saúl
AU - Martínez-Palomo, Adolfo
AU - Tsutsumi, Víctor
AU - Shibayama, Mineko
PY - 2009/6
Y1 - 2009/6
N2 - The human large intestine can harbor two morphologically similar amoebae; the invasive Entamoeba histolytica and the non-invasive Entamoeba dispar. Whereas E. histolytica can produce intestinal and extra-intestinal lesions, E. dispar is present in non-symptomatic carriers. Although biochemical, genetic and proteomic studies have identified clear differences between these Entamoebae, it has become clear that several molecules, once assumed to be involved in tissue destruction, exist in both the virulent and the avirulent species. As surface molecules may play a role in invasion and could therefore determine which amoebae are invasive, we analyzed the glycoconjugate composition of E. histolytica and E. dispar using lectins. There was a significant difference between E. histolytica and E. dispar in the expression of glycoconjugates containing d-mannose and N-acetyl-α-d-galactosamine residues, but not between virulent and avirulent strains of E. histolytica. N-glycoconjugates with terminal α (1-3)-linked mannose residues participate in the adhesion and subsequent cytotoxicity of E. histolytica to cultured hamster hepatocytes. One of them probably is the Gal/GalNAc lectin.
AB - The human large intestine can harbor two morphologically similar amoebae; the invasive Entamoeba histolytica and the non-invasive Entamoeba dispar. Whereas E. histolytica can produce intestinal and extra-intestinal lesions, E. dispar is present in non-symptomatic carriers. Although biochemical, genetic and proteomic studies have identified clear differences between these Entamoebae, it has become clear that several molecules, once assumed to be involved in tissue destruction, exist in both the virulent and the avirulent species. As surface molecules may play a role in invasion and could therefore determine which amoebae are invasive, we analyzed the glycoconjugate composition of E. histolytica and E. dispar using lectins. There was a significant difference between E. histolytica and E. dispar in the expression of glycoconjugates containing d-mannose and N-acetyl-α-d-galactosamine residues, but not between virulent and avirulent strains of E. histolytica. N-glycoconjugates with terminal α (1-3)-linked mannose residues participate in the adhesion and subsequent cytotoxicity of E. histolytica to cultured hamster hepatocytes. One of them probably is the Gal/GalNAc lectin.
KW - Entamoeba dispar
KW - Entamoeba histolytica
KW - Glycoconjugates
KW - Protozoa
KW - Surface properties
KW - Virulence
UR - http://www.scopus.com/inward/record.url?scp=67349098551&partnerID=8YFLogxK
U2 - 10.1016/j.parint.2009.02.003
DO - 10.1016/j.parint.2009.02.003
M3 - Artículo
C2 - 19269346
SN - 1383-5769
VL - 58
SP - 171
EP - 177
JO - Parasitology International
JF - Parasitology International
IS - 2
ER -