TY - JOUR
T1 - Dietary fulvic acid effects on survival and expression of immune-related genes in Litopenaeus vannamei challenged with Vibrio parahaemolyticus
AU - Fierro-Coronado, Jesús A.
AU - Angulo, Carlos
AU - Rubio-Castro, Arturo
AU - Luna-González, Antonio
AU - Cáceres-Martínez, Carlos J.
AU - Ruiz-Verdugo, Cesar A.
AU - Álvarez-Ruíz, Píndaro
AU - Escamilla-Montes, Ruth
AU - González-Ocampo, Héctor A.
AU - Diarte-Plata, Genaro
N1 - Publisher Copyright:
© 2018 John Wiley & Sons Ltd
PY - 2018/9
Y1 - 2018/9
N2 - The effects of fulvic acid (FA) on survival and immune-related gene expression were investigated in Litopenaeus vannamei challenged with Vibrio parahaemolyticus by immersion. Shrimp were fed with different dietary FA concentrations (1, 2, 4 and 6 g/kg feed) for 20 days (first bioassay) or 8 days (second bioassay, 2 g/kg feed of FA added every 2 days) and then challenged with V. parahaemolyticus. In a third bioassay, the expression of three immune-related genes (translationally controlled tumour protein [TCTP], superoxide dismutase [SOD] and heat-shock protein 70 [HSP70]) in haemocytes or hepatopancreas of experimental shrimp was measured by real-time quantitative PCR at 0, 6, 12, 24, 48, 72 and 96 hr after FA (2 g/kg feed) administration. Fulvic acid increased survival at a concentration of 2 g/kg feed supplied every two days. Interestingly, TCTP gene expression was upregulated, whereas gene expression of SOD and HSP70 was downregulated. In conclusion, dietary fulvic acid improves survival in white shrimp challenged with V. parahaemolyticus and modulates the immune response. Therefore, FA merits further evaluation as prophylactic treatment in commercial shrimp farms.
AB - The effects of fulvic acid (FA) on survival and immune-related gene expression were investigated in Litopenaeus vannamei challenged with Vibrio parahaemolyticus by immersion. Shrimp were fed with different dietary FA concentrations (1, 2, 4 and 6 g/kg feed) for 20 days (first bioassay) or 8 days (second bioassay, 2 g/kg feed of FA added every 2 days) and then challenged with V. parahaemolyticus. In a third bioassay, the expression of three immune-related genes (translationally controlled tumour protein [TCTP], superoxide dismutase [SOD] and heat-shock protein 70 [HSP70]) in haemocytes or hepatopancreas of experimental shrimp was measured by real-time quantitative PCR at 0, 6, 12, 24, 48, 72 and 96 hr after FA (2 g/kg feed) administration. Fulvic acid increased survival at a concentration of 2 g/kg feed supplied every two days. Interestingly, TCTP gene expression was upregulated, whereas gene expression of SOD and HSP70 was downregulated. In conclusion, dietary fulvic acid improves survival in white shrimp challenged with V. parahaemolyticus and modulates the immune response. Therefore, FA merits further evaluation as prophylactic treatment in commercial shrimp farms.
KW - AHPND
KW - Litopenaeus vannamei
KW - Vibrio parahaemolyticus
KW - fulvic acid
KW - gene expression
UR - http://www.scopus.com/inward/record.url?scp=85050608691&partnerID=8YFLogxK
U2 - 10.1111/are.13789
DO - 10.1111/are.13789
M3 - Artículo
SN - 1355-557X
VL - 49
SP - 3218
EP - 3227
JO - Aquaculture Research
JF - Aquaculture Research
IS - 9
ER -