TY - JOUR
T1 - Development of 24 tetra-nucleotide microsatellite markers in Cortes Geoduck Panopea globosa by next-generation sequencing
AU - Cruz-Hernández, Pedro
AU - Munguia-Vega, Adrian
AU - Leyva-Valencia, Ignacio
AU - Lucero-Burquez, Fátima
AU - Lluch-Cota, Daniel B.
N1 - Funding Information:
Acknowledgments A.M.V. received support from the PANGAS project and The David & Lucile Packard Foundation. Sergio Gon-zález, Edgar Alcántara (CIBNOR), Sergio Pérez (CEDO) and Laura Pérez provided technical help with sampling. The research was funded by Consejo Nacional de Ciencia y Tecnología (CONACYT, Grant 106905).
PY - 2014/8
Y1 - 2014/8
N2 - Panopea globosa is a marine bivalve mollusk important for small-scale fisheries from Northwest Mexico. Twenty-four tetra-nucleotide microsatellite markers were characterized by 454 XL+ Titanium pyrosequencing. High average levels of allelic diversity and heterozygosity were observed (NA = 16.68; Ne = 9.77; HO = 0.643; HE = 0.858). Significant deviations from HWE, as previously observed in other mollusks, were detected in twelve loci. Linkage disequilibrium was detected among two loci pairs. This set of microsatellite loci will be useful to determine the genetic structure and larval connectivity of P. globosa.
AB - Panopea globosa is a marine bivalve mollusk important for small-scale fisheries from Northwest Mexico. Twenty-four tetra-nucleotide microsatellite markers were characterized by 454 XL+ Titanium pyrosequencing. High average levels of allelic diversity and heterozygosity were observed (NA = 16.68; Ne = 9.77; HO = 0.643; HE = 0.858). Significant deviations from HWE, as previously observed in other mollusks, were detected in twelve loci. Linkage disequilibrium was detected among two loci pairs. This set of microsatellite loci will be useful to determine the genetic structure and larval connectivity of P. globosa.
KW - Gulf of California
KW - Marine connectivity
KW - Next generation sequencing
KW - Panopea
KW - Tetra-nucleotide microsatellite
UR - http://www.scopus.com/inward/record.url?scp=84905643947&partnerID=8YFLogxK
U2 - 10.1007/s12686-014-0172-1
DO - 10.1007/s12686-014-0172-1
M3 - Artículo
SN - 1877-7252
VL - 6
SP - 531
EP - 533
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 3
ER -