Determination of lytic activity of bacteriophages in rhizospheric samples from alfalfa plantation

Agriculture is a fundamental activity for human development, one of the most important fodder crops in the world is alfalfa, for a good production some edaphic characteristics are required and know to improve the yield of the crop, as well as rhizospheric microorganisms and knowing them improves crop yield. Derived from the commercial demand, the health, quality and safety requirements of the crops are increased, which leads to the search for new care strategies before phytopathogenic organisms that avoid lowering the quality of the product. In recent years, bacteriophages have been studied as a biological control which act specifically against bacteria causing lysis of these. In this work, the edaphic characteristics of alfalfa cultivation soil in Santa Marta Etla, Oaxaca, were evaluated; demonstrating that they are appropriate for crop growth; Bacteriophages were identified by means of soil filtrates and challenging their lythic activity in rhizospheric bacteria isolated from the same study soil which presented high host amplitude for them, in addition to effectiveness against the phytopathogenic bacteria Pseudomonas phaseoli, Clavibacter michiganensis, Xanthomonas axonopodis pv. phaseoli, Xanthomonas campestris and Pseudomonas syringae, so they have a potential use as biological control in crops infected by these microorganisms, reducing the harmful effects on the flora, fauna, population and the environment.