TY - JOUR
T1 - Detection of white spot syndrome virus (WSSV) in the Pacific oyster Crassostrea gigas
AU - Vazquez-Boucard, Celia
AU - Alvarez-Ruiz, Pindaro
AU - Escobedo-Fregoso, Cristina
AU - Anguiano-Vega, Gerardo
AU - Duran-Avelar, Ma de Jesus
AU - Pinto, Vania Serrano
AU - Escobedo-Bonilla, César Marcial
N1 - Funding Information:
Support for this project came from Consejo Nacional de Ciencia y Tecnologia (CONACYT Grant 60030/2006), Comites de Sanidad Acuicola from Sonora (COSAES), Nayarit (CESANAY), Sinaloa (CESASIN), and Baja California Sur (CSABCS). Special thanks to G. Lara at CEDI LAB, P. Parrilla, and G. de la Fuente for their help during this study. This work is part of the Innovation Consortium for the Competitiveness of the Aquaculture Sector.
PY - 2010/7
Y1 - 2010/7
N2 - Oysters Crassostrea gigas were placed at water supply canals of three shrimp farms in Guasave, Mexico where WSSV outbreaks occur. Animals were sampled through April-August and September-December to detect WSSV DNA. By using three different PCR protocols, only oysters from a farm undergoing a WSSV outbreak were found WSSV-positive in gills and digestive gland. Two WSSV amplicons were sequenced and they corresponded over 99% to WSSV genome segments. Results showed that oysters can capture WSSV particles suspended in water. Susceptibility of oysters to WSSV infection and their role as a carrier remain to be determined.
AB - Oysters Crassostrea gigas were placed at water supply canals of three shrimp farms in Guasave, Mexico where WSSV outbreaks occur. Animals were sampled through April-August and September-December to detect WSSV DNA. By using three different PCR protocols, only oysters from a farm undergoing a WSSV outbreak were found WSSV-positive in gills and digestive gland. Two WSSV amplicons were sequenced and they corresponded over 99% to WSSV genome segments. Results showed that oysters can capture WSSV particles suspended in water. Susceptibility of oysters to WSSV infection and their role as a carrier remain to be determined.
KW - Crassostrea gigas
KW - Digestive gland
KW - Filter-feeding
KW - Gills
KW - Plankton
KW - Pond water
KW - WSSV
UR - http://www.scopus.com/inward/record.url?scp=77954865676&partnerID=8YFLogxK
U2 - 10.1016/j.jip.2010.04.004
DO - 10.1016/j.jip.2010.04.004
M3 - Artículo
C2 - 20398672
SN - 0022-2011
VL - 104
SP - 245
EP - 247
JO - Journal of Invertebrate Pathology
JF - Journal of Invertebrate Pathology
IS - 3
ER -