TY - JOUR
T1 - Detection of Biomarkers Associated with Acute Kidney Injury by a Gold Nanoparticle Based Colloidal Nano-Immunosensor by Fourier-Transform Infrared Spectroscopy with Principal Component Analysis
AU - Ortega-Hernández, Noelia
AU - Ortega-Romero, Manolo
AU - Medeiros-Domingo, Mara
AU - Barbier, Olivier Christophe
AU - Rojas-López, Marlon
N1 - Publisher Copyright:
© 2022 Taylor & Francis Group, LLC.
PY - 2022
Y1 - 2022
N2 - A colloidal nano-immunosensor based on gold nanoparticles covered in their surface with protein A and the immunoglobulin anti-kidney injury molecule-1 was prepared and tested in human urine to identify a biomarker that diagnose acute kidney injury. The urine was obtained from patients with cancer treated with cisplatin, diagnosed with acute kidney injury, and from healthy individuals. Fourier-transform infrared spectroscopy analysis showed important spectral differences between both types of groups, primarily for the intervals from 1750 to 1500 cm−1 and from 1000 to 891 cm−1 where the amide I group (C = O) and the glycosylated functional groups of this protein absorb. The principal component analysis applied to the vibrational signals obtained with the nano-immunosensor allows adequate discrimination of urine from patients with acute kidney injury from samples from healthy individuals. The simplicity of the proposed method, from the preparation of the nano-immunosensor to the spectral and chemometric analysis, may make this method an interesting alternative to practically diagnose acute kidney injury using a small volume of urine.
AB - A colloidal nano-immunosensor based on gold nanoparticles covered in their surface with protein A and the immunoglobulin anti-kidney injury molecule-1 was prepared and tested in human urine to identify a biomarker that diagnose acute kidney injury. The urine was obtained from patients with cancer treated with cisplatin, diagnosed with acute kidney injury, and from healthy individuals. Fourier-transform infrared spectroscopy analysis showed important spectral differences between both types of groups, primarily for the intervals from 1750 to 1500 cm−1 and from 1000 to 891 cm−1 where the amide I group (C = O) and the glycosylated functional groups of this protein absorb. The principal component analysis applied to the vibrational signals obtained with the nano-immunosensor allows adequate discrimination of urine from patients with acute kidney injury from samples from healthy individuals. The simplicity of the proposed method, from the preparation of the nano-immunosensor to the spectral and chemometric analysis, may make this method an interesting alternative to practically diagnose acute kidney injury using a small volume of urine.
KW - Acute kidney injury
KW - Fourier-transform infrared (FTIR) spectroscopy
KW - gold nanoparticles
KW - nano immunosensor
KW - principal component analysis (PCA)
UR - http://www.scopus.com/inward/record.url?scp=85127276233&partnerID=8YFLogxK
U2 - 10.1080/00032719.2022.2053982
DO - 10.1080/00032719.2022.2053982
M3 - Artículo
AN - SCOPUS:85127276233
SN - 0003-2719
VL - 55
SP - 2370
EP - 2381
JO - Analytical Letters
JF - Analytical Letters
IS - 15
ER -