TY - JOUR
T1 - Design of a portable luminescence bio-tool for on-site analysis of heavy metals in water samples
AU - Mishra, Rupesh K.
AU - Rhouati, Amina
AU - Bueno, Diana
AU - Anwar, Muhammad Waqas
AU - Shahid, Shakir Ahmad
AU - Sharma, Vinay
AU - Marty, Jean Louis
AU - Hayat, Akhtar
N1 - Publisher Copyright:
© 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2018/9/26
Y1 - 2018/9/26
N2 - In this work, we report an innovative tool for heavy metal screening in water samples. This new chemiluminescent set-up screens the light generated from luminol oxidation by horseradish peroxidase (HRP) in the presence of hydrogen peroxide (H2O2). The pollutant concentrations in real water samples were calculated by studying the effect of metal ions on chemiluminescence signal. Owing to its simplicity, portability and low cost, this approach presents a real alternative to classical optical methods. It is constructed with simple materials: a black box containing a cuvette and a micro-camera. When the enzymatic reaction takes place, the luminescence is captured by the camera placed in upright position. The image can be saved automatically in a computer for further analysis using a MATLAB interface. The RGB diagram is then established to determine the analyte concentrations in the tested samples. This method was successfully applied for the determination of mercury (Hg), lead (Pb) and cadmium (Cd) in lake and field water samples. In these experiments, three concentrations of each analytes were tested (5, 25 and 50 µg/L). We noted a good proportionality between the analyte concentration and the chemiluminescent detection intensity. Detection of binary and tertiary combinations of heavy metals has been also investigated. The developed biosensor showed low detection limits for the tested heavy metals: 1, 0.7 and 0.02 for Hg2+, Pb2+ and Cd2+, respectively. Finally, excellent recoveries ranging from 98% to 104% were obtained for the HRP-inhibition assay.
AB - In this work, we report an innovative tool for heavy metal screening in water samples. This new chemiluminescent set-up screens the light generated from luminol oxidation by horseradish peroxidase (HRP) in the presence of hydrogen peroxide (H2O2). The pollutant concentrations in real water samples were calculated by studying the effect of metal ions on chemiluminescence signal. Owing to its simplicity, portability and low cost, this approach presents a real alternative to classical optical methods. It is constructed with simple materials: a black box containing a cuvette and a micro-camera. When the enzymatic reaction takes place, the luminescence is captured by the camera placed in upright position. The image can be saved automatically in a computer for further analysis using a MATLAB interface. The RGB diagram is then established to determine the analyte concentrations in the tested samples. This method was successfully applied for the determination of mercury (Hg), lead (Pb) and cadmium (Cd) in lake and field water samples. In these experiments, three concentrations of each analytes were tested (5, 25 and 50 µg/L). We noted a good proportionality between the analyte concentration and the chemiluminescent detection intensity. Detection of binary and tertiary combinations of heavy metals has been also investigated. The developed biosensor showed low detection limits for the tested heavy metals: 1, 0.7 and 0.02 for Hg2+, Pb2+ and Cd2+, respectively. Finally, excellent recoveries ranging from 98% to 104% were obtained for the HRP-inhibition assay.
KW - Portable device
KW - heavy metals
KW - luminescence assay
KW - on-site analysis
KW - water samples
UR - http://www.scopus.com/inward/record.url?scp=85053695947&partnerID=8YFLogxK
U2 - 10.1080/03067319.2018.1521395
DO - 10.1080/03067319.2018.1521395
M3 - Artículo
SN - 0306-7319
VL - 98
SP - 1081
EP - 1094
JO - International Journal of Environmental Analytical Chemistry
JF - International Journal of Environmental Analytical Chemistry
IS - 12
ER -