TY - JOUR
T1 - Degradation of polychlorinated biphenyl (PCB) by a consortium obtained from a contaminated soil composed of Brevibacterium, Pandoraea and Ochrobactrum
AU - Liz, Juan Antonio Zermeño Eguia
AU - Jan-Roblero, Janet
AU - De La Serna, Javier Zavala Díaz
AU - De León, Arturo Vera Ponce
AU - Hernández-Rodríguez, César
N1 - Funding Information:
Acknowledgments This work was supported by grants SIP20070651 and SIP20070164 Instituto Politécnico Nacional (IPN). C. Hernández-Rodríguez and J. Jan-Roblero appreciate the fellowship of Comisiónde Operación y Fomento de Actividades Académicas (COFAA) and Estímulo al Desempeño Académico (EDI), IPN, and Sistema Nacional de Investigadores (SNI), CONACyT.
PY - 2009/1
Y1 - 2009/1
N2 - An indigenous polychlorinated biphenyl (PCB)-degrading bacterial consortium was obtained from soils contaminated by transformer oil with a high content of PCBs. The PCB degrader strains were isolated and identified as Brevibacterium antarcticum, Pandoraea pnomenusa, and Ochrobactrum intermedium by 16S rRNA gene sequence phylogenetic analysis. The PCB-degrading ability of the consortium and of individual strains was determined by using GC/MS. The PCB-degrading capacities of the consortium were evaluated for three concentrations of transfomer oil ranging from 55 to 152 μM supplemented with 0.001% biphenyl and 0.1% of Tween 80 surfactant. PCB biodegradation by the consortium was favored in the presence of both additives and the greatest extent of biodegradation (67.5%) was obtained at a PCB concentration of 55 μM. Each bacterial species exhibited a particular pattern of degradation relating to specific PCB congeners. Isolated strains showed a moderate degradation capability towards tetra-, hepta-, and octa-chlorobiphenyls; although no effect on penta-, hexa-, and nona-chlorobiphenyls was observed. Recently, PCB degradation capacity was recognized in a Pandorea member; however, this is the first study that describes the ability of Brevibacterium and Ochrobactrum species to degrade PCBs.
AB - An indigenous polychlorinated biphenyl (PCB)-degrading bacterial consortium was obtained from soils contaminated by transformer oil with a high content of PCBs. The PCB degrader strains were isolated and identified as Brevibacterium antarcticum, Pandoraea pnomenusa, and Ochrobactrum intermedium by 16S rRNA gene sequence phylogenetic analysis. The PCB-degrading ability of the consortium and of individual strains was determined by using GC/MS. The PCB-degrading capacities of the consortium were evaluated for three concentrations of transfomer oil ranging from 55 to 152 μM supplemented with 0.001% biphenyl and 0.1% of Tween 80 surfactant. PCB biodegradation by the consortium was favored in the presence of both additives and the greatest extent of biodegradation (67.5%) was obtained at a PCB concentration of 55 μM. Each bacterial species exhibited a particular pattern of degradation relating to specific PCB congeners. Isolated strains showed a moderate degradation capability towards tetra-, hepta-, and octa-chlorobiphenyls; although no effect on penta-, hexa-, and nona-chlorobiphenyls was observed. Recently, PCB degradation capacity was recognized in a Pandorea member; however, this is the first study that describes the ability of Brevibacterium and Ochrobactrum species to degrade PCBs.
KW - Brevibacterium antarcticum
KW - Ochrobactrum intermedium
KW - Pandoraea pnomenusa
KW - Polychlorinated biphenyls
UR - http://www.scopus.com/inward/record.url?scp=57349110661&partnerID=8YFLogxK
U2 - 10.1007/s11274-008-9875-3
DO - 10.1007/s11274-008-9875-3
M3 - Artículo
SN - 0959-3993
VL - 25
SP - 165
EP - 170
JO - World Journal of Microbiology and Biotechnology
JF - World Journal of Microbiology and Biotechnology
IS - 1
ER -