TY - JOUR
T1 - Degradation of benzene, toluene, and xylene isomers by a bacterial consortium obtained from rhizosphere soil of Cyperus sp. grown in a petroleum-contaminated area
AU - Ortega-González, Diana Katherine
AU - Zaragoza, Diego
AU - Aguirre-Garrido, José
AU - Ramírez-Saad, Hugo
AU - Hernández-Rodríguez, César
AU - Jan-Roblero, Janet
N1 - Funding Information:
Acknowledgments This work was supported by grants SIP20110709 and SIP20120324 from the Instituto Politécnico Nacional (IPN). D.K. Ortega-González gives thanks to CONACyT and the Programa Institucional de Formación de Investigadores (PIFI), IPN, for scholarships. J. Jan-Roblero and C. Hernández-Rodríguez appreciate the COFAA, and EDI, IPN fellowships and support from the SNI and CONACyT. Furthermore, we would like to thank J.C. Cancino-Díaz, E. Tao Wang Hu, and E. Cristiani for critical reading of the manuscript.
PY - 2013/11
Y1 - 2013/11
N2 - Increasing contamination of soil and groundwater with benzene, toluene, and xylene (BTX) due to activities of the chemical and oil refinery industry has caused serious environmental damage. Efficient methods are required to isolate and degrade them. Microorganisms associated with rhizosphere soil are considered efficient agents to remediate hydrocarbon contamination. In this study, we obtained a stabilized bacterial consortium from the rhizosphere soil of Cyperus sp. grown in a petroleum-contaminated field in Southern Mexico. This consortium was able to completely degrade BTX in 14 days. Bacteria isolated from the consortium were identified by 16S rRNA gene sequence analysis as Ralstonia insidiosa, Cellulomonas hominis, Burkholderia kururiensis, and Serratia marcescens. The BTX-degradation capacity of the bacterial consortium was confirmed by the detection of genes pheA, todC1, and xylM, which encoded phenol hydroxylase, toluene 1,2-dioxygenase, and xylene monooxygenase, respectively. Our results demonstrate feasibility of BTX biodegradation by indigenous bacteria that might be used for soil remediation in Southern Mexico.
AB - Increasing contamination of soil and groundwater with benzene, toluene, and xylene (BTX) due to activities of the chemical and oil refinery industry has caused serious environmental damage. Efficient methods are required to isolate and degrade them. Microorganisms associated with rhizosphere soil are considered efficient agents to remediate hydrocarbon contamination. In this study, we obtained a stabilized bacterial consortium from the rhizosphere soil of Cyperus sp. grown in a petroleum-contaminated field in Southern Mexico. This consortium was able to completely degrade BTX in 14 days. Bacteria isolated from the consortium were identified by 16S rRNA gene sequence analysis as Ralstonia insidiosa, Cellulomonas hominis, Burkholderia kururiensis, and Serratia marcescens. The BTX-degradation capacity of the bacterial consortium was confirmed by the detection of genes pheA, todC1, and xylM, which encoded phenol hydroxylase, toluene 1,2-dioxygenase, and xylene monooxygenase, respectively. Our results demonstrate feasibility of BTX biodegradation by indigenous bacteria that might be used for soil remediation in Southern Mexico.
UR - http://www.scopus.com/inward/record.url?scp=84884673975&partnerID=8YFLogxK
U2 - 10.1007/s12223-013-0248-4
DO - 10.1007/s12223-013-0248-4
M3 - Artículo
C2 - 23564628
SN - 0015-5632
VL - 58
SP - 569
EP - 577
JO - Folia Microbiologica
JF - Folia Microbiologica
IS - 6
ER -