TY - JOUR
T1 - Defense gene expression in root galls induced by Nacobbus aberrans in CM334 chilli plants
AU - Villar-Luna, E.
AU - García-Espinoza, J. A.
AU - Goméz-Rodriguez, O.
AU - Rojas-Martínez, R. I.
AU - Zavaleta-Mejía, E.
N1 - Publisher Copyright:
© 2015 Sciendo. All rights reserved.
PY - 2015/2/2
Y1 - 2015/2/2
N2 - Capsicum annuum L. CM334 is susceptible to Nacobbus aberrans but highly resistant to Phytophthora capsici. Resistance to P. capsici is associated with the over-expression of various defense genes such as those encoding pathogenesis-related proteins. The transcriptional alterations of defense-related genes were determined in galls induced by N. aberrans (Na) in CM334 chili roots. Transcripts accumulation of WRKY-a, WRKY1, POX (peroxidase), PR-1 (pathogenesis-related protein 1), and EAS (5-epiaristolochene synthase) was estimated by qRT-PCR, and they were compared with those recorded in the incompatible CM334- P. capsici (Pc) interaction. The levels of all studied genes were significantly (P ≤ 0.05) lower (WRKY1, POX and PR-1) or down-regulated (WRKY-a and EAS) in the presence of N. aberrans; in contrast, in the incompatible interaction, all genes were significantly up-regulated. The alterations induced by N. aberrans could be necessary to ensure the successful completion of its life cycle in CM334 chili roots.
AB - Capsicum annuum L. CM334 is susceptible to Nacobbus aberrans but highly resistant to Phytophthora capsici. Resistance to P. capsici is associated with the over-expression of various defense genes such as those encoding pathogenesis-related proteins. The transcriptional alterations of defense-related genes were determined in galls induced by N. aberrans (Na) in CM334 chili roots. Transcripts accumulation of WRKY-a, WRKY1, POX (peroxidase), PR-1 (pathogenesis-related protein 1), and EAS (5-epiaristolochene synthase) was estimated by qRT-PCR, and they were compared with those recorded in the incompatible CM334- P. capsici (Pc) interaction. The levels of all studied genes were significantly (P ≤ 0.05) lower (WRKY1, POX and PR-1) or down-regulated (WRKY-a and EAS) in the presence of N. aberrans; in contrast, in the incompatible interaction, all genes were significantly up-regulated. The alterations induced by N. aberrans could be necessary to ensure the successful completion of its life cycle in CM334 chili roots.
KW - Capsicum annuum
KW - Gene reprogramming
KW - QRT-PCR
KW - Transcription factors
UR - http://www.scopus.com/inward/record.url?scp=85091650650&partnerID=8YFLogxK
U2 - 10.1515/helmin-2015-0014
DO - 10.1515/helmin-2015-0014
M3 - Artículo
AN - SCOPUS:85091650650
SN - 0440-6605
VL - 52
SP - 77
EP - 82
JO - Helminthologia (Poland)
JF - Helminthologia (Poland)
IS - 1
ER -