TY - JOUR
T1 - Cytotoxic effect of the immunotoxin constructed of the ribosome-inactivating protein curcin and the monoclonal antibody against Her2 receptor on tumor cells
AU - Jaramillo-Quintero, Lidia Patricia
AU - De Oca, Arturo Contis Montes
AU - Rojas, Andrés Romero
AU - Rojas-Hernández, Saúl
AU - Campos-Rodríguez, Rafael
AU - Martínez-Ayala, Alma Leticia
N1 - Publisher Copyright:
© 2015 Japan Society for Bioscience, Biotechnology, and Agrochemistry.
PY - 2015
Y1 - 2015
N2 - The toxicity of the curcin on cancer cells allows to consider this protein as the toxic component of an immunotoxin directed to Her2, which is associated with cancer. Reductive amination was proposed to conjugate curcin and an anti-Her2; the binding was tested using Polyacrylamide gel electrophoresis, western blot, and immunocytochemistry. The in vitro cytotoxicity of curcin and the immunotoxin was assessed on breast cancer cell lines SK-BR-3 (Her2+) and MDA-MB-231 (Her2-). IC50 values for curcin were 15.5 ± 8.3 and 18.6 ± 2.4 μg/mL, respectively, statistically equivalent (p < 0.05). While to the immunotoxin was 2.2 ± 0.08 for SK-BR-3 and 147.6 ± 2.5 μg/mL for MDA-MB-231. These values showed that the immunotoxin was seven times more toxic to the SK-BR-3 than curcin and eight times less toxic to the MDA-MB-231. The immunotoxin composed of curcin and an antibody against Her2 and constructed by reductive amination could be a therapeutic candidate against Her2+ cancer.
AB - The toxicity of the curcin on cancer cells allows to consider this protein as the toxic component of an immunotoxin directed to Her2, which is associated with cancer. Reductive amination was proposed to conjugate curcin and an anti-Her2; the binding was tested using Polyacrylamide gel electrophoresis, western blot, and immunocytochemistry. The in vitro cytotoxicity of curcin and the immunotoxin was assessed on breast cancer cell lines SK-BR-3 (Her2+) and MDA-MB-231 (Her2-). IC50 values for curcin were 15.5 ± 8.3 and 18.6 ± 2.4 μg/mL, respectively, statistically equivalent (p < 0.05). While to the immunotoxin was 2.2 ± 0.08 for SK-BR-3 and 147.6 ± 2.5 μg/mL for MDA-MB-231. These values showed that the immunotoxin was seven times more toxic to the SK-BR-3 than curcin and eight times less toxic to the MDA-MB-231. The immunotoxin composed of curcin and an antibody against Her2 and constructed by reductive amination could be a therapeutic candidate against Her2+ cancer.
KW - Breast cancer
KW - Chemical conjugation
KW - ErBB2
KW - Reductive amination
KW - Targeted therapy
UR - http://www.scopus.com/inward/record.url?scp=84940038124&partnerID=8YFLogxK
U2 - 10.1080/09168451.2015.1006572
DO - 10.1080/09168451.2015.1006572
M3 - Artículo
C2 - 25704287
SN - 0916-8451
VL - 79
SP - 896
EP - 906
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 6
ER -