TY - JOUR
T1 - Culture studies of Alexandrium affine (Dinophyceae), a non-toxic cyst forming dinoflagellate from Bahía Concepción, Gulf of California
AU - Band-Schmidt, C. J.
AU - Lechuga-Devéze, C. H.
AU - Kulis, D. M.
AU - Anderson, D. M.
N1 - Funding Information:
The authors wish to thank B. Keafer for his assistance in germination experiments, the CIBNOR Graduate Program and the National Council of Science and Technology (CONACyT) for the financial and logistical support, F. Hernández-Sandoval, L. Morquecho, I. Murillo-Murillo, J. Ramírez, and F. Zapata-Vázquez for their assistance during the field work; L. Morquecho is also thanked for use of the digital camera; I. Fogel for English language editing. This research was partially supported by CIBNOR Institutional Research Projects AYCG-8 and AYCG-11 (1999 – 2000). This work was also supported by NOAA Grant No. NA96OP0099 and NSF Grant No. OCE-9808173 (to DMA). This is contribution number 65 of the U.S. ECOHAB Program sponsored by NOAA, EPA, NSF, NASA, and ONR and grant contribution 10763 from the Woods Hole Oceanographic Institution.
PY - 2003/1
Y1 - 2003/1
N2 - Alexandrium affine (Inoue et Fukuyo) Balech, isolated from Bahía Concepción (Gulf of California), was studied to determine the effect of environmental factors on cyst germination and vegetative growth. Alexandrium affine was homothallic and isogamous, and formed cysts in nutrient-deficient (N- or P-limiting) medium. The maturation period of newly formed cysts varied between two weeks and three months, depending on the storage temperature, with colder temperatures prolonging the process. The rate of germination increased with increasing temperature, and was not significantly influenced by light. Germination experiments suggest a broad temperature window for A. affine cysts, ranging from 5 to 25 °C. The optimal vegetative growth rates were 0.25 to 0.34 day-1 at 20-30 °C. No vegetative growth was observed below 15 °C or above 35 °C. With HPLC toxin analyses, we confirm that this species does not produce saxitoxins. These data on the dormancy, excystment, and growth characteristics seem to be regulated by the environmental constraints of this subtropical bay.
AB - Alexandrium affine (Inoue et Fukuyo) Balech, isolated from Bahía Concepción (Gulf of California), was studied to determine the effect of environmental factors on cyst germination and vegetative growth. Alexandrium affine was homothallic and isogamous, and formed cysts in nutrient-deficient (N- or P-limiting) medium. The maturation period of newly formed cysts varied between two weeks and three months, depending on the storage temperature, with colder temperatures prolonging the process. The rate of germination increased with increasing temperature, and was not significantly influenced by light. Germination experiments suggest a broad temperature window for A. affine cysts, ranging from 5 to 25 °C. The optimal vegetative growth rates were 0.25 to 0.34 day-1 at 20-30 °C. No vegetative growth was observed below 15 °C or above 35 °C. With HPLC toxin analyses, we confirm that this species does not produce saxitoxins. These data on the dormancy, excystment, and growth characteristics seem to be regulated by the environmental constraints of this subtropical bay.
UR - http://www.scopus.com/inward/record.url?scp=0037285043&partnerID=8YFLogxK
U2 - 10.1515/BOT.2003.007
DO - 10.1515/BOT.2003.007
M3 - Artículo
SN - 0006-8055
VL - 46
SP - 44
EP - 54
JO - Botanica Marina
JF - Botanica Marina
IS - 1
ER -