TY - JOUR
T1 - Crosstalk between circadian rhythmicity, mitochondrial dynamics and macrophage bactericidal activity
AU - Oliva-Ramírez, Jacqueline
AU - Moreno-Altamirano, María Maximina B.
AU - Pineda-Olvera, Benjamín
AU - Cauich-Sánchez, Patricia
AU - Javier Sánchez-García, F.
N1 - Publisher Copyright:
© 2014 John Wiley & Sons Ltd.
PY - 2014
Y1 - 2014
N2 - Biological functions show rhythmic fluctuations with 24-hr periodicity regulated by circadian proteins encoded by the so-called 'clock' genes. The absence or deregulation of circadian proteins in mice leads to metabolic disorders and in vitro models have shown that the synthesis of proinflammatory cytokines by macrophages follows a circadian rhythm so showing a link between circadian rhythmicity, metabolism and immunity. Recent evidence reveals that mitochondrial shape, position and size, collectively referred to as mitochondrial dynamics, are related to both cell metabolism and immune function. However, studies addressing the simultaneous crosstalk between circadian rhythm, mitochondrial dynamics and cell immune function are scarce. Here, by using an in vitro model of synchronized murine peritoneal macrophages, we present evidence that the mitochondrial dynamics and the mitochondrial membrane potential (ΔΨm) follow a circadian rhythmic pattern. In addition, it is shown that the fusion of mitochondria along with high ΔΨm, indicative of high mitochondrial activity, precede the highest phagocytic and bactericidal activity of macrophages on Salmonella typhimurium. Taken together, our results suggest a timely coordination between circadian rhythmicity, mitochondrial dynamics, and the bactericidal capacity of macrophages.
AB - Biological functions show rhythmic fluctuations with 24-hr periodicity regulated by circadian proteins encoded by the so-called 'clock' genes. The absence or deregulation of circadian proteins in mice leads to metabolic disorders and in vitro models have shown that the synthesis of proinflammatory cytokines by macrophages follows a circadian rhythm so showing a link between circadian rhythmicity, metabolism and immunity. Recent evidence reveals that mitochondrial shape, position and size, collectively referred to as mitochondrial dynamics, are related to both cell metabolism and immune function. However, studies addressing the simultaneous crosstalk between circadian rhythm, mitochondrial dynamics and cell immune function are scarce. Here, by using an in vitro model of synchronized murine peritoneal macrophages, we present evidence that the mitochondrial dynamics and the mitochondrial membrane potential (ΔΨm) follow a circadian rhythmic pattern. In addition, it is shown that the fusion of mitochondria along with high ΔΨm, indicative of high mitochondrial activity, precede the highest phagocytic and bactericidal activity of macrophages on Salmonella typhimurium. Taken together, our results suggest a timely coordination between circadian rhythmicity, mitochondrial dynamics, and the bactericidal capacity of macrophages.
KW - Bactericidal activity
KW - Circadian rhythmicity
KW - Macrophages
KW - Mitochondrial dynamics
UR - http://www.scopus.com/inward/record.url?scp=84908284127&partnerID=8YFLogxK
U2 - 10.1111/imm.12329
DO - 10.1111/imm.12329
M3 - Artículo
C2 - 24903615
SN - 0019-2805
VL - 143
SP - 490
EP - 497
JO - Immunology
JF - Immunology
IS - 3
ER -