TY - JOUR
T1 - Congo Red affects the growth, morphology and activity of glucosamine-6-phosphate synthase in the human pathogenic fungus Sporothrix schenckii
AU - Sánchez-López, Juan Francisco
AU - González-Ibarra, Joaquín
AU - Macías-Segoviano, Juan Ignacio
AU - Cuéllar-Cruz, Mayra
AU - Álvarez-Vargas, Aurelio
AU - Cano-Canchola, Carmen
AU - López-Romero, Everardo
N1 - Publisher Copyright:
© 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Sporothrixschenckii is the etiological agent of sporotrichosis, a mycosis of humans and other mammals. Little is known about the responses of this thermodimorphic pathogen to perturbations in the cell wall (CW) by different stress conditions. Here we describe the effect of Congo Red (CR) on the fungal growth, morphogenesis and activity of glucosamine-6-phosphate (GlcN-6-P) synthase. Under conditions of yeast development, 15 µM CR abolished conidia (CN) germination, but when yeast cells were first obtained in the absence of the dye and then post-incubated in its presence, yeasts rapidly differentiated into mycelial cells. On the other hand, under conditions of mycelium development, 150 µM CR did not affect CN germination, but filamentous cells underwent structural changes characterized by a distorted CW contour, the loss of polarity and the formation of red-pigmented, hyphal globose structures. Under these conditions, CR also induced a significant and transient increase in the activity of GlcN-6-P synthase, an essential enzyme in CW biogenesis.
AB - Sporothrixschenckii is the etiological agent of sporotrichosis, a mycosis of humans and other mammals. Little is known about the responses of this thermodimorphic pathogen to perturbations in the cell wall (CW) by different stress conditions. Here we describe the effect of Congo Red (CR) on the fungal growth, morphogenesis and activity of glucosamine-6-phosphate (GlcN-6-P) synthase. Under conditions of yeast development, 15 µM CR abolished conidia (CN) germination, but when yeast cells were first obtained in the absence of the dye and then post-incubated in its presence, yeasts rapidly differentiated into mycelial cells. On the other hand, under conditions of mycelium development, 150 µM CR did not affect CN germination, but filamentous cells underwent structural changes characterized by a distorted CW contour, the loss of polarity and the formation of red-pigmented, hyphal globose structures. Under these conditions, CR also induced a significant and transient increase in the activity of GlcN-6-P synthase, an essential enzyme in CW biogenesis.
KW - Congo Red
KW - Fungal responses
KW - Sporothrix schenckii
UR - http://www.scopus.com/inward/record.url?scp=85055035031&partnerID=8YFLogxK
U2 - 10.1007/s00203-018-1576-1
DO - 10.1007/s00203-018-1576-1
M3 - Artículo
C2 - 30302500
AN - SCOPUS:85055035031
SN - 0302-8933
VL - 201
SP - 135
EP - 141
JO - Archives of Microbiology
JF - Archives of Microbiology
IS - 1
ER -