TY - JOUR
T1 - Complete genome sequence of a velogenic newcastle disease virus isolated in Mexico
AU - Absalón, Angel E.
AU - Mariano-Matías, Andrea
AU - Vásquez-Márquez, Alejandra
AU - Morales-Garzón, Andrés
AU - Cortés-Espinosa, Diana V.
AU - Ortega-García, Roberto
AU - Lucio-Decanini, Eduardo
N1 - Funding Information:
Acknowledgments We would like to thank Dr. Claudio L. Afonso for reviewing the manuscript. This study was supported by CONA-CYT (Grant Salud-2009-C02-126990) and by the Instituto Politecnico Nacional (Grand SIP-20121834).
PY - 2012/10
Y1 - 2012/10
N2 - In Mexico, the number of cases of the highly virulent Newcastle disease virus is increasing. In 2005, an outbreak of Newcastle disease occurred on an egg laying hen farm in the state of Puebla despite vaccination with the LaSota strain. Farmers experienced a major drop in egg production as a consequence of a field challenge virus. In this study, we characterize the virus, APMV1/chicken/ Mexico/P05/2005, responsible for the outbreak. The virus is categorized as a velogenic virus with an intracranial pathogenicity index of 1.99 and a chicken embryo mean death time of 36 h. The complete genome length of the virus was sequenced as consisting of 15,192 bp. In addition, phylogenetic analysis classified the virus as a member of the class II, genotype V. The highly pathogenic nature of the virus has been linked to the amino acid sequence at the fusion protein cleavage site, which contains multiple basic amino acids (RRQKR;F).
AB - In Mexico, the number of cases of the highly virulent Newcastle disease virus is increasing. In 2005, an outbreak of Newcastle disease occurred on an egg laying hen farm in the state of Puebla despite vaccination with the LaSota strain. Farmers experienced a major drop in egg production as a consequence of a field challenge virus. In this study, we characterize the virus, APMV1/chicken/ Mexico/P05/2005, responsible for the outbreak. The virus is categorized as a velogenic virus with an intracranial pathogenicity index of 1.99 and a chicken embryo mean death time of 36 h. The complete genome length of the virus was sequenced as consisting of 15,192 bp. In addition, phylogenetic analysis classified the virus as a member of the class II, genotype V. The highly pathogenic nature of the virus has been linked to the amino acid sequence at the fusion protein cleavage site, which contains multiple basic amino acids (RRQKR;F).
KW - Avian paramyxovirus
KW - Genetic analysis
KW - Mexico
KW - Newcastle disease virus
KW - Velogenic
UR - http://www.scopus.com/inward/record.url?scp=84868151493&partnerID=8YFLogxK
U2 - 10.1007/s11262-012-0782-1
DO - 10.1007/s11262-012-0782-1
M3 - Artículo
C2 - 22821201
SN - 0920-8569
VL - 45
SP - 304
EP - 310
JO - Virus Genes
JF - Virus Genes
IS - 2
ER -