Resumen
Background: Epigenetic modifications are key factors modulating the expression of genes involved in the
synthesis of phytochemicals. The knowledge of plant epigenetic and genetic variations can contribute to
enhance the production of bioactive compounds. These issues have been little explored thus far in Rorippa
nasturtium var. aquaticum L. (watercress), an edible and medicinal plant. The aim of the current study was to
determine and compare the phenolic composition and epigenetic and genetic variations between wild and
cultivated watercress.
Results: Significant differenceswere found in the quantitative phenolic composition betweenwild and cultivated
watercress. The eight primer combinations used in the methylation-sensitive amplification polymorphism
(MSAP) method revealed different epigenetic status for each watercress type, the cultivated one being the
most epigenetically variable. The genetic variability revealed by the EcoRI/MspI amplification profile and also
by eight inter-simple sequence repeat (ISSR) primers was different between the two types of watercress. The
results of the Mantel test showed that the correlation between genetic and epigenetic variations has
diminished in the cultivated type. Cluster analyses showed that the epigenetic and genetic characterizations
clearly discriminated between wild and cultivated watercress.
Conclusions: Relevant chemical, epigenetic, and genetic differences have emerged between wild and cultivated
watercress. These differences can contribute to fingerprint and develop quality control tools for the integral
and safety use and the commercialization of watercress. The richness of epialleles could support the
development of tools to manipulate the watercress epigenome to develop high bioproduct–producing cultivars.
How to cite: Gutiérrez-Velázquez MV, Almaraz-Abarca N, Herrera-Arrieta Y, et al. Comparison of the phenolic
contents and the epigenetic and genetic variability of wild and cultivated watercress (Rorippa nasturtium var.
aquaticum L.).
synthesis of phytochemicals. The knowledge of plant epigenetic and genetic variations can contribute to
enhance the production of bioactive compounds. These issues have been little explored thus far in Rorippa
nasturtium var. aquaticum L. (watercress), an edible and medicinal plant. The aim of the current study was to
determine and compare the phenolic composition and epigenetic and genetic variations between wild and
cultivated watercress.
Results: Significant differenceswere found in the quantitative phenolic composition betweenwild and cultivated
watercress. The eight primer combinations used in the methylation-sensitive amplification polymorphism
(MSAP) method revealed different epigenetic status for each watercress type, the cultivated one being the
most epigenetically variable. The genetic variability revealed by the EcoRI/MspI amplification profile and also
by eight inter-simple sequence repeat (ISSR) primers was different between the two types of watercress. The
results of the Mantel test showed that the correlation between genetic and epigenetic variations has
diminished in the cultivated type. Cluster analyses showed that the epigenetic and genetic characterizations
clearly discriminated between wild and cultivated watercress.
Conclusions: Relevant chemical, epigenetic, and genetic differences have emerged between wild and cultivated
watercress. These differences can contribute to fingerprint and develop quality control tools for the integral
and safety use and the commercialization of watercress. The richness of epialleles could support the
development of tools to manipulate the watercress epigenome to develop high bioproduct–producing cultivars.
How to cite: Gutiérrez-Velázquez MV, Almaraz-Abarca N, Herrera-Arrieta Y, et al. Comparison of the phenolic
contents and the epigenetic and genetic variability of wild and cultivated watercress (Rorippa nasturtium var.
aquaticum L.).
| Título traducido de la contribución | Comparación del contenido fenólico y la variabilidad genética y epigenética de Rorippa nasturtium var. aquaticum L.sivestre y cultivada |
|---|---|
| Idioma original | Inglés |
| Páginas (desde-hasta) | 9 |
| Número de páginas | 16 |
| Publicación | Electronic Journal of Biotechnology |
| Volumen | 34 |
| Estado | Publicada - 30 abr. 2018 |
Palabras clave
- Bioactive compounds Bioproducts Brassicaceae Cytosine methylation Edible plants Epigenetic mechanism Epigenetic modifications Epigenome