TY - JOUR
T1 - Comparison of chito-oligosaccharide production from three different colloidal chitosans using the endochitonsanolytic system of Bacillus thuringiensis
AU - Olicón-Hernández, Dario Rafael
AU - Vázquez-Landaverde, Pedro Alberto
AU - Cruz-Camarillo, Ramón
AU - Rojas-Avelizapa, Luz Irene
N1 - Publisher Copyright:
© 2017 Taylor & Francis.
PY - 2017/2/7
Y1 - 2017/2/7
N2 - Bacillus thuringiensis is a nonhuman pathogen bacterium that is used as a fungal and insect biocontrol agent. Because of its environmental interaction, it possesses several extracellular enzymes that are able to degrade chitin and chitosan, two of the most important polymers because of their application in numerous fields. However, in recent years, it has been observed that oligosaccharides from the enzymatic degradation of chitosan have important benefits for human health. Comparison and exploration of the production of chito-oligosaccharides from different sources of chitosan will improve the process parameters and expand the biotechnology based in these molecules. This study shows the production of chito-oligosaccharides from three different sources of colloidal chitosan and conducts a qualitative–quantitative comparison between them, using the extracellular enzyme of B. thuringiensis. We found that in the three substrates, it is possible to get a mixture of chito-oligosaccharides from dimer to hexamer in a concentration range from 0.72 to 8.09 mg · g−1 of original substrate. The best substrate to obtain these molecules was commercial chitosan as it has the highest production yields.
AB - Bacillus thuringiensis is a nonhuman pathogen bacterium that is used as a fungal and insect biocontrol agent. Because of its environmental interaction, it possesses several extracellular enzymes that are able to degrade chitin and chitosan, two of the most important polymers because of their application in numerous fields. However, in recent years, it has been observed that oligosaccharides from the enzymatic degradation of chitosan have important benefits for human health. Comparison and exploration of the production of chito-oligosaccharides from different sources of chitosan will improve the process parameters and expand the biotechnology based in these molecules. This study shows the production of chito-oligosaccharides from three different sources of colloidal chitosan and conducts a qualitative–quantitative comparison between them, using the extracellular enzyme of B. thuringiensis. We found that in the three substrates, it is possible to get a mixture of chito-oligosaccharides from dimer to hexamer in a concentration range from 0.72 to 8.09 mg · g−1 of original substrate. The best substrate to obtain these molecules was commercial chitosan as it has the highest production yields.
KW - Bacillus thuringiensis
KW - chitin
KW - chito-oligosaccharides
KW - chitosan
KW - chitosanolytic activity
KW - shrimp waste
UR - http://www.scopus.com/inward/record.url?scp=84997820925&partnerID=8YFLogxK
U2 - 10.1080/10826068.2016.1181086
DO - 10.1080/10826068.2016.1181086
M3 - Artículo
C2 - 27830993
AN - SCOPUS:84997820925
SN - 1082-6068
VL - 47
SP - 116
EP - 122
JO - Preparative Biochemistry and Biotechnology
JF - Preparative Biochemistry and Biotechnology
IS - 2
ER -