TY - JOUR
T1 - Combined use of novel chitosan-grafted N-hydroxyethyl acrylamide polyurethane and human dermal fibroblasts as a construct for in vitro-engineered skin
AU - González-Torres, Maykel
AU - Elizalde-Cárdenas, Alejandro
AU - Leyva-Gómez, Gerardo
AU - González-Mendoza, Oswaldo
AU - Lima, Enrique
AU - Núñez-Tapia, Israel
AU - Abad-Contreras, David Eduardo
AU - Luisa Del Prado-Audelo, María
AU - Pichardo-Bahena, Raúl
AU - Carlos-Martínez, Alberto
AU - Ribas-Aparicio, Rosa María
N1 - Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/5/31
Y1 - 2023/5/31
N2 - A rich plethora of information about grafted chitosan (CS) for medical use has been reported. The capability of CS-grafted poly(N-hydroxyethyl acrylamide) (CS-g-PHEAA) to support human dermal fibroblasts (HDFs) in vitro has been proven. However, CS-grafted copolymers lack good stiffness and the characteristic microstructure of a cellular matrix. In addition, whether CS-g-PHEAA can be used to prepare a scaffold with a suitable morphology and mechanical properties for skin tissue engineering (STE) is unclear. This study aimed to show for the first time that step-growth polymerizations can be used to obtain polyurethane (PU) platforms of CS-g-PHEAA, which can also have enhanced microhardness and be suitable for in vitro cell culture. The PU prepolymers were prepared from grafted CS, polyethylene glycol, and 1,6-hexamethylene diisocyanate. The results proved that a poly(saccharide-urethane) [(CS-g-PHEAA)-PU] could be successfully synthesized with a more suitable microarchitecture, thermal properties, and topology than CS-PU for the dynamic culturing of fibroblasts. Cytotoxicity, proliferation, histological and immunophenotype assessments revealed significantly higher biocompatibility and cell proliferation of the derivative concerning the controls. Cells cultured on (CS-g-PHEAA)-PU displayed a quiescent state compared to those cultured on CS-PU, which showed an activated phenotype. These findings may be critical factors in future studies establishing wound dressing models.
AB - A rich plethora of information about grafted chitosan (CS) for medical use has been reported. The capability of CS-grafted poly(N-hydroxyethyl acrylamide) (CS-g-PHEAA) to support human dermal fibroblasts (HDFs) in vitro has been proven. However, CS-grafted copolymers lack good stiffness and the characteristic microstructure of a cellular matrix. In addition, whether CS-g-PHEAA can be used to prepare a scaffold with a suitable morphology and mechanical properties for skin tissue engineering (STE) is unclear. This study aimed to show for the first time that step-growth polymerizations can be used to obtain polyurethane (PU) platforms of CS-g-PHEAA, which can also have enhanced microhardness and be suitable for in vitro cell culture. The PU prepolymers were prepared from grafted CS, polyethylene glycol, and 1,6-hexamethylene diisocyanate. The results proved that a poly(saccharide-urethane) [(CS-g-PHEAA)-PU] could be successfully synthesized with a more suitable microarchitecture, thermal properties, and topology than CS-PU for the dynamic culturing of fibroblasts. Cytotoxicity, proliferation, histological and immunophenotype assessments revealed significantly higher biocompatibility and cell proliferation of the derivative concerning the controls. Cells cultured on (CS-g-PHEAA)-PU displayed a quiescent state compared to those cultured on CS-PU, which showed an activated phenotype. These findings may be critical factors in future studies establishing wound dressing models.
KW - Biopolymers
KW - Chitosan
KW - Fibroblast
KW - N-hydroxyethyl acrylamide
KW - Polymer synthesis
UR - http://www.scopus.com/inward/record.url?scp=85150897294&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2023.124136
DO - 10.1016/j.ijbiomac.2023.124136
M3 - Artículo
C2 - 36965555
AN - SCOPUS:85150897294
SN - 0141-8130
VL - 238
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
M1 - 124136
ER -