TY - JOUR
T1 - Combined administration routes of marine yeasts enhanced immune-related genes and protection of white shrimp (Penaeus vannamei) against Vibrio parahaemolyticus
AU - Licona-Jain, Alan
AU - Racotta, Ilie
AU - Angulo, Carlos
AU - Luna-González, Antonio
AU - Escamilla-Montes, Ruth
AU - Cortés-Jacinto, Edilmar
AU - Morelos-Castro, Rosa M.
AU - Campa-Córdova, Ángel I.
N1 - Publisher Copyright:
© 2022
Copyright © 2022 Elsevier Ltd. All rights reserved.
PY - 2022/5
Y1 - 2022/5
N2 - Antibiotic usage to control infectious diseases in shrimp aquaculture has led to serious problems on antimicrobial resistance. An alternative to mitigate this issue is the use of probiotics, which can be easily administered by feed and water. This study examines immunomodulatory and protective effects of the marine yeasts Debaryomyces hansenii CBS8339 (Dh) and Yarrowia lipolytica Yl-N6 (Yl) -alone and mixed-in white shrimp Penaeus vannamei post-larvae. Administration routes (fed and water alone or in combination), supplementation frequency and time elapsed after the last dietary supplement were tested on growth and gene expression of penaeidin, lectin, lysozyme, superoxide dismutase, catalase, and peroxidase, as well as survival upon Vibrio parahaemolyticus IPNGS16 challenge. Penaeidin and lectin genes were upregulated in post-larvae fed orally with Yl or combined Dh + Yl. Higher growth and survival for yeast supplementation treatments were observed compared to the control group, mainly when yeasts (Dh + Yl) and administration routes (feed and water) were combined. In conclusion, mixed yeast and combined administration routes improved growth and immunity against V. parahaemolyticus.
AB - Antibiotic usage to control infectious diseases in shrimp aquaculture has led to serious problems on antimicrobial resistance. An alternative to mitigate this issue is the use of probiotics, which can be easily administered by feed and water. This study examines immunomodulatory and protective effects of the marine yeasts Debaryomyces hansenii CBS8339 (Dh) and Yarrowia lipolytica Yl-N6 (Yl) -alone and mixed-in white shrimp Penaeus vannamei post-larvae. Administration routes (fed and water alone or in combination), supplementation frequency and time elapsed after the last dietary supplement were tested on growth and gene expression of penaeidin, lectin, lysozyme, superoxide dismutase, catalase, and peroxidase, as well as survival upon Vibrio parahaemolyticus IPNGS16 challenge. Penaeidin and lectin genes were upregulated in post-larvae fed orally with Yl or combined Dh + Yl. Higher growth and survival for yeast supplementation treatments were observed compared to the control group, mainly when yeasts (Dh + Yl) and administration routes (feed and water) were combined. In conclusion, mixed yeast and combined administration routes improved growth and immunity against V. parahaemolyticus.
KW - Aquaculture
KW - Crustacean
KW - Disease resistance
KW - Pathogen
KW - Probiotic
KW - Water
KW - Vibrio parahaemolyticus
KW - Immunity, Innate/genetics
KW - Penaeidae
KW - Animal Feed/analysis
KW - Animals
KW - Diet
KW - Lectins/pharmacology
UR - http://www.scopus.com/inward/record.url?scp=85127798899&partnerID=8YFLogxK
U2 - 10.1016/j.fsi.2022.04.004
DO - 10.1016/j.fsi.2022.04.004
M3 - Artículo
C2 - 35398528
AN - SCOPUS:85127798899
SN - 1050-4648
VL - 124
SP - 192
EP - 200
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
ER -