TY - JOUR
T1 - CLSM and TIRF images from lignocellulosic materials
T2 - Garlic skin and agave fibers study
AU - Hernández-Varela, Josué
AU - Chanona-Pérez, José
AU - Benavides, Héctor Calderón
AU - Cerda, Susana Gallegos
AU - Victoriano, Lizbeth Gonzalez
AU - de Jesús Perea Flores, María
AU - López, Maximiliano Campos
AU - Candelas, Liliana Edith Rojas
AU - Tamato, Benjamín Arrendondo
N1 - Publisher Copyright:
© Microscopy Society of America 2021.
PY - 2021
Y1 - 2021
N2 - Fluorescence techniques have been widely used by scientists to reveal valuable information from biological samples, but in food science, small progress is known due to the complexity of the samples. In this study, two different biological samples, garlic skin (GS) and agave fibers (AF), were used to evaluate the techniques of confocal laser scanning microscopy (CLSM) and total internal reflection fluorescence (TIRF) microscopy, to obtain valuable information on the fiber size of the samples. A compositional characterization with calcofluor white in CLSM was achieved, but a superficial characterization of the samples with TIRF was made, evidencing fiber sizes of 398.67 ± 48.47 nm and 677.38 ± 76.88 nm for GS and AF, respectively. This work reveals that only an untreated sample can be used with the two techniques in the same microscope. In addition, it is possible to characterize the sample only using a spatial field of research and which valuable information about the structure of the material is found. This work provides the opportunity to use advanced fluorescence techniques for elucidation of structures shortly before studied with these techniques.
AB - Fluorescence techniques have been widely used by scientists to reveal valuable information from biological samples, but in food science, small progress is known due to the complexity of the samples. In this study, two different biological samples, garlic skin (GS) and agave fibers (AF), were used to evaluate the techniques of confocal laser scanning microscopy (CLSM) and total internal reflection fluorescence (TIRF) microscopy, to obtain valuable information on the fiber size of the samples. A compositional characterization with calcofluor white in CLSM was achieved, but a superficial characterization of the samples with TIRF was made, evidencing fiber sizes of 398.67 ± 48.47 nm and 677.38 ± 76.88 nm for GS and AF, respectively. This work reveals that only an untreated sample can be used with the two techniques in the same microscope. In addition, it is possible to characterize the sample only using a spatial field of research and which valuable information about the structure of the material is found. This work provides the opportunity to use advanced fluorescence techniques for elucidation of structures shortly before studied with these techniques.
UR - http://www.scopus.com/inward/record.url?scp=85118659120&partnerID=8YFLogxK
U2 - 10.1017/S1431927621006334
DO - 10.1017/S1431927621006334
M3 - Artículo
AN - SCOPUS:85118659120
SN - 1431-9276
VL - 27
SP - 1730
EP - 1734
JO - Microscopy and Microanalysis
JF - Microscopy and Microanalysis
IS - 1
ER -