TY - JOUR
T1 - Chronic toxicity of shrimp feed added with silver nanoparticles (Argovit-4r) in Litopenaeus vannamei and immune response to white spot syndrome virus infection
AU - Romo Quiñonez, Carlos R.
AU - Alvarez-Ruiz, Píndaro
AU - Mejía-Ruiz, Claudio H.
AU - Bogdanchikova, Nina
AU - Pestryakov, Alexey
AU - Gamez-Jimenez, Carina
AU - Valenzuela-Quiñonez, Wenceslao
AU - Montoya-Mejía, Magnolia
AU - Pérez, Eusebio Nava
N1 - Publisher Copyright:
© 2022 PeerJ Inc.. All rights reserved.
PY - 2022/11/22
Y1 - 2022/11/22
N2 - In recent years, the application of silver nanoparticles (AgNPs) as antibacterial compounds has been widely used in human and veterinary medicine. In this work, we investigated the effects of AgNPs (Argovit-4 R) as feed additives (feed-AgNPs) on shrimp (Litopenaeus vannamei) using three different methods: 1) chronic toxicity after 28 days of feeding, 2) Effects against white spot syndrome virus (WSSV) challenged by oral route, and 3) transcriptional responses of immune-related genes (PAP, ProPO, CTL-3, Crustin, PEN3, and PEN4) following WSSV infection. The results showed that the feed-AgNPs did not interfere with the growth and survival of shrimp. Also, mild lesions in the hepatopancreas were recorded, proportional to the frequency of the feed-AgNP supply. Challenge test versus WSSV showed that feeding every 7 days with feed-AgNPs reduced mortality, reaching a survival rate of 53%, compared to the survival rates observed in groups fed every 4 days, daily and control groups of feed-AgNPs for the 30%, 10%, and 7% groups, respectively. Feed-AgNPs negatively regulated the expression of PAP, ProPO, and Crustin genes after 28 days of treatment and altered the transcriptional responses of PAP, ProPO, CTL-3, and Crustin after WSSV exposure. The results showed that weekly feeding-AgNPs could partially prevent WSSV infection in shrimp culture. However, whether or not transcriptional responses against pathogens are advantageous remains to be elucidated.
AB - In recent years, the application of silver nanoparticles (AgNPs) as antibacterial compounds has been widely used in human and veterinary medicine. In this work, we investigated the effects of AgNPs (Argovit-4 R) as feed additives (feed-AgNPs) on shrimp (Litopenaeus vannamei) using three different methods: 1) chronic toxicity after 28 days of feeding, 2) Effects against white spot syndrome virus (WSSV) challenged by oral route, and 3) transcriptional responses of immune-related genes (PAP, ProPO, CTL-3, Crustin, PEN3, and PEN4) following WSSV infection. The results showed that the feed-AgNPs did not interfere with the growth and survival of shrimp. Also, mild lesions in the hepatopancreas were recorded, proportional to the frequency of the feed-AgNP supply. Challenge test versus WSSV showed that feeding every 7 days with feed-AgNPs reduced mortality, reaching a survival rate of 53%, compared to the survival rates observed in groups fed every 4 days, daily and control groups of feed-AgNPs for the 30%, 10%, and 7% groups, respectively. Feed-AgNPs negatively regulated the expression of PAP, ProPO, and Crustin genes after 28 days of treatment and altered the transcriptional responses of PAP, ProPO, CTL-3, and Crustin after WSSV exposure. The results showed that weekly feeding-AgNPs could partially prevent WSSV infection in shrimp culture. However, whether or not transcriptional responses against pathogens are advantageous remains to be elucidated.
KW - AgNP
KW - Aquaculture
KW - Argovit
KW - Chronic toxicity
KW - Litopenaeus vannamei
KW - Shrimp
KW - Silver fed
KW - Silver nanoparticles
KW - WSSV
KW - White spot syndrome virus
UR - http://www.scopus.com/inward/record.url?scp=85143744997&partnerID=8YFLogxK
U2 - 10.7717/peerj.14231
DO - 10.7717/peerj.14231
M3 - Artículo
C2 - 36438583
AN - SCOPUS:85143744997
SN - 2167-8359
VL - 10
JO - PeerJ
JF - PeerJ
M1 - e14231
ER -