TY - JOUR
T1 - Chitosan resistance by the deletion of the putative high affinity glucose transporter in the yeast Ustilago maydis
AU - Olicón-Hernández, Dario Rafael
AU - Araiza-Villanueva, Minerva Georgina
AU - Vázquez-Carrada, Melissa
AU - Guerra-Sánchez, Guadalupe
N1 - Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/7
Y1 - 2021/7
N2 - Chitosan is a polycationic amino-sugar polymer soluble in acidic pH. As a potential antifungal, it has been tested against several fungi. Its main mode of action is the permeabilization of cell membrane by the interaction with specific membrane sites. Ustilago maydis, an attractive fungal model used in biochemical and biotechnology research, is highly sensitive to chitosan, with extensive membrane destruction that results in cell death. Using the Golden Gate system, several mutant strains with deletions in monosaccharide transporters were obtained and tested against chitosan in order to know the implications of these membrane proteins in the sensitivity of the fungus against chitosan. Δum11514/03895 strain, a mutant with a deletion in a hypothetical high affinity glucose transporter, showed resistance to chitosan. Morphological characterization of the mutant displayed an apparent increase in mitochondrial content, but oxygen consumption as well as growth rate were not affected by the gene deletion. Alteration in cell wall surface was observed in the mutant strain. In contrast to wild type, the Δum11514/03895 strain showed integrity of cell wall and cell membrane in the presence of chitosan. The resistance against chitosan is likely associated to the modification of cell wall architecture and is not related to energy-depend process.
AB - Chitosan is a polycationic amino-sugar polymer soluble in acidic pH. As a potential antifungal, it has been tested against several fungi. Its main mode of action is the permeabilization of cell membrane by the interaction with specific membrane sites. Ustilago maydis, an attractive fungal model used in biochemical and biotechnology research, is highly sensitive to chitosan, with extensive membrane destruction that results in cell death. Using the Golden Gate system, several mutant strains with deletions in monosaccharide transporters were obtained and tested against chitosan in order to know the implications of these membrane proteins in the sensitivity of the fungus against chitosan. Δum11514/03895 strain, a mutant with a deletion in a hypothetical high affinity glucose transporter, showed resistance to chitosan. Morphological characterization of the mutant displayed an apparent increase in mitochondrial content, but oxygen consumption as well as growth rate were not affected by the gene deletion. Alteration in cell wall surface was observed in the mutant strain. In contrast to wild type, the Δum11514/03895 strain showed integrity of cell wall and cell membrane in the presence of chitosan. The resistance against chitosan is likely associated to the modification of cell wall architecture and is not related to energy-depend process.
KW - Antifungal mechanism
KW - Chitosan
KW - Glucose transporter
KW - Ustilago maydis
UR - http://www.scopus.com/inward/record.url?scp=85105571741&partnerID=8YFLogxK
U2 - 10.1016/j.carres.2021.108335
DO - 10.1016/j.carres.2021.108335
M3 - Artículo
C2 - 33989946
AN - SCOPUS:85105571741
SN - 0008-6215
VL - 505
JO - Carbohydrate Research
JF - Carbohydrate Research
M1 - 108335
ER -