TY - JOUR
T1 - Chemoprotective effect of Spirulina (Arthrospira) against cyclophosphamide-induced mutagenicity in mice
AU - Chamorro-Cevallos, G.
AU - Garduño-Siciliano, L.
AU - Barrón, B. L.
AU - Madrigal-Bujaidar, E.
AU - Cruz-Vega, D. E.
AU - Pages, N.
N1 - Funding Information:
This work was supported in part by the grant 200708555, SIP, I.P.N., México; G.C., L.C., B.L.B. and E.M.B are fellows of the EDI, EDD and COFAA/IPN Programs.
PY - 2008/2
Y1 - 2008/2
N2 - The aim of this study was to investigate the antimutagenic effects of Spirulina (SP) on male and female mice by the dominant lethal test using cyclophosphamide (CP) as a mutagen. Animals of both sex were given SP orally at 0, 200, 400 or 800 mg/kg body weight (b.w.) for 2 weeks prior to starting the CP treatment. CP was i.p. injected daily for 5 days at 40 mg/kg b.w. For the male-dominant lethal test, each male was caged with untreated females per week for 3 weeks. For the female-dominant lethal test the above doses and schedule treatments were used and treated females were caged for one week with untreated males (1-2). On days 13-15 after breeding was |started all the females were evaluated for incidence of pregnancy, total corpora lutea, total implants and pre- and post-implant losses. In the male-dominant lethal test, the CP induced pre- and post-implant losses in untreated females were inhibited at all SP doses. In the female-dominant lethal test only post-implantation losses were prevented at the same doses. Semen examination of a separate group of mice showed that SP improved its quality. Our results illustrate protective effects of SP in relation to CP-induced genetic damage to germ cells.
AB - The aim of this study was to investigate the antimutagenic effects of Spirulina (SP) on male and female mice by the dominant lethal test using cyclophosphamide (CP) as a mutagen. Animals of both sex were given SP orally at 0, 200, 400 or 800 mg/kg body weight (b.w.) for 2 weeks prior to starting the CP treatment. CP was i.p. injected daily for 5 days at 40 mg/kg b.w. For the male-dominant lethal test, each male was caged with untreated females per week for 3 weeks. For the female-dominant lethal test the above doses and schedule treatments were used and treated females were caged for one week with untreated males (1-2). On days 13-15 after breeding was |started all the females were evaluated for incidence of pregnancy, total corpora lutea, total implants and pre- and post-implant losses. In the male-dominant lethal test, the CP induced pre- and post-implant losses in untreated females were inhibited at all SP doses. In the female-dominant lethal test only post-implantation losses were prevented at the same doses. Semen examination of a separate group of mice showed that SP improved its quality. Our results illustrate protective effects of SP in relation to CP-induced genetic damage to germ cells.
KW - Antigenotoxicity
KW - Cyclophosphamide
KW - Dominant lethal mutation
KW - Spirulina
UR - http://www.scopus.com/inward/record.url?scp=37449019325&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2007.08.039
DO - 10.1016/j.fct.2007.08.039
M3 - Artículo
SN - 0278-6915
VL - 46
SP - 567
EP - 574
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
IS - 2
ER -