TY - JOUR
T1 - Chemical and functional changes of human insulin by in vitro incubation with blood from diabetic patients in oxidative stress
AU - Montes-Cortes, Daniel H.
AU - Hicks, Juan J.
AU - Ceballos-Reyes, Guillermo M.
AU - Garcia-Sanchez, Jose R.
AU - Medina-Navarro, Rafael
AU - Olivares-Corichi, Ivonne M.
N1 - Funding Information:
This work was supported by CONACYT CB-2006-01-57570 and IPN-ESM-SIP 20062107, 20071407, 20080303, and 20082978.
PY - 2010/7
Y1 - 2010/7
N2 - Oxidative stress damage to biomolecules has been implicated in several diseases including diabetes mellitus. In the present study, we investigated the effect of oxidative stress in whole blood (WB) from diabetic patients (n = 60) on recombinant human insulin. Insulin was incubated with WB obtained from diabetic patients (DP) who had hyperglycemia (>300 mg/dL) or from 41 healthy volunteers (HV). Whole blood of DP, unlike WB of HV, induced higher values of formazan (142%), dityrosines (279%), and carbonyls (58%) in the insulin residues. Interestingly, the insulin modified by WB of DP showed less hypoglycemic activity in rat (30%) in comparison with insulin incubated with WB of HV. The incubation of insulin in WB from DP induces chemical changes in insulin and a decrease in its biological activity, events that might be associated with the high levels of oxidative stress markers found in the plasma of these patients.
AB - Oxidative stress damage to biomolecules has been implicated in several diseases including diabetes mellitus. In the present study, we investigated the effect of oxidative stress in whole blood (WB) from diabetic patients (n = 60) on recombinant human insulin. Insulin was incubated with WB obtained from diabetic patients (DP) who had hyperglycemia (>300 mg/dL) or from 41 healthy volunteers (HV). Whole blood of DP, unlike WB of HV, induced higher values of formazan (142%), dityrosines (279%), and carbonyls (58%) in the insulin residues. Interestingly, the insulin modified by WB of DP showed less hypoglycemic activity in rat (30%) in comparison with insulin incubated with WB of HV. The incubation of insulin in WB from DP induces chemical changes in insulin and a decrease in its biological activity, events that might be associated with the high levels of oxidative stress markers found in the plasma of these patients.
UR - http://www.scopus.com/inward/record.url?scp=77952942473&partnerID=8YFLogxK
U2 - 10.1016/j.metabol.2009.10.013
DO - 10.1016/j.metabol.2009.10.013
M3 - Artículo
C2 - 20022071
SN - 0026-0495
VL - 59
SP - 935
EP - 942
JO - Metabolism: Clinical and Experimental
JF - Metabolism: Clinical and Experimental
IS - 7
ER -