Chelativorans petroleitrophicus sp. nov., a paraffin oil-degrading bacterium isolated from a mixture of oil-based drill cuttings and paddy soil

Jinyang Yu, Hanjun Liu, Yonggang Deng, Petri Penttinen, Zuyu Wang, Yihao Liu, Yan Wang, Entao Wang, Yu Sun, Keming Chen, Hui Li, Liyun Chen, Xiumei Yu, Ke Zhao, Yunfu Gu, Min Huang, Lingzi Zhang, Quanju Xiang, Menggen Ma, Qiang Chen

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Resumen

We isolated a paraffin oil-degrading bacterial strain from a mixture of oil-based drill cutting and paddy soil, and characterized the strain using a polyphasic approach. The Gram-positive, aerobic, rod-shaped and non-spore-forming strain (SCAU 2101T) grew optimally at 50 °C, pH 7.0 and 0.5% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence indicated that the strain represented a distinct clade in the genus Chelativorans, neighbouring Chelativorans intermedius LMG 28482T (97.1 %). The genome size and DNA G+C content of the strain were 3 969 430 bp and 63.1 mol%, respectively. Whole genome based phylogenomic analyses showed that the average nucleotide identity and digital DNA–DNA hybridization values between strain SCAU 2101T and C. intermedius LMG 28482T were 77.5 and 21.2%, respectively. The major respiratory quinone was Q-10. The dominant fatty acids were C19:0 cyclo ω8c (50.6%), summed feature 8 (C18:1 ω7c and/or C18:1 ω6c; 22.5%) and C18:0 (13.8%). The polar lipids of the strain included phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, phosphatidylcholine and diphosphatidylglycerol. Based on the results, strain SCAU 2101T was considered to represent a novel species in the genus Chelativorans, for which the name Chelativorans petroleitrophicus sp. nov. is proposed. The type strain is SCAU 2101T (= CCTCC AB 2021125T=KCTC 92067T).

Idioma originalInglés
Número de artículo005769
PublicaciónInternational Journal of Systematic and Evolutionary Microbiology
Volumen73
N.º3
DOI
EstadoPublicada - 1 mar. 2023
Publicado de forma externa

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