TY - JOUR
T1 - Characterization of microsatellite loci from two-spotted octopus Octopus bimaculatus Verrill 1883 from pyrosequencing reads
AU - Domínguez-Contreras, J. F.
AU - Munguía-Vega, A.
AU - Ceballos-Vázquez, B. P.
AU - Arellano-Martínez, M.
AU - Culver, M.
N1 - Funding Information:
Acknowledgments We thank staff from Pronatura Noroeste A. C., Comunidad y Biodiversidad A. C. and the Intercultural Center for the Study of Deserts and Oceans (CEDO A. C.), Funding was provided by The David and Lucile Packard Foundation via the PANGAS project (http://pangas.arizona.edu), the Western Society of Malacologists, and the SIP projects: 20120971, 20121594, 20130059, 20130089, CONACyT 108230. JFDC receive scholarships from CONACyT and PIFI-IPN and the results here presented are part of his Ph.D dissertation.
PY - 2014/6
Y1 - 2014/6
N2 - We characterized 22 novel microsatellite loci in the two-spotted octopus Octopus bimaculatus using 454 pyrosequencing reads. All loci were polymorphic and will be used in studies of marine connectivity aimed at increasing sustainability of the resource. The mean number alleles per locus was 13.09 (range 7-19) and observed heterozygosities ranged from 0.50 to 1.00. Four loci pairs were linked and three deviated from Hardy-Weinberg equilibrium. Eighteen and 12 loci were polymorphic in Octopus bimaculoides and Octopus hubbsorum, respectively.
AB - We characterized 22 novel microsatellite loci in the two-spotted octopus Octopus bimaculatus using 454 pyrosequencing reads. All loci were polymorphic and will be used in studies of marine connectivity aimed at increasing sustainability of the resource. The mean number alleles per locus was 13.09 (range 7-19) and observed heterozygosities ranged from 0.50 to 1.00. Four loci pairs were linked and three deviated from Hardy-Weinberg equilibrium. Eighteen and 12 loci were polymorphic in Octopus bimaculoides and Octopus hubbsorum, respectively.
KW - Gulf of California
KW - Marine connectivity
KW - Mexican Pacific
KW - Next-generation sequencing
KW - Octopus
UR - http://www.scopus.com/inward/record.url?scp=84900821512&partnerID=8YFLogxK
U2 - 10.1007/s12686-013-0128-x
DO - 10.1007/s12686-013-0128-x
M3 - Carta
SN - 1877-7252
VL - 6
SP - 465
EP - 468
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 2
ER -
