TY - JOUR
T1 - Cell metabolic changes of porphyrins and superoxide anions by anthracene and benzo(a)pyrene
AU - Uribe-Hernández, Raúl
AU - Pérez-Zapata, Aura J.
AU - Vega-Barrita, María L.
AU - Ramón-Gallegos, Eva
AU - Amezcua-Allieri, Myriam A.
N1 - Funding Information:
Financial and material support for this study was provided by Instituto Mexicano del Petróleo and Instituto Politécnico Nacional through the D.0888 and CGPI-20060663 projects, respectively. E. Ramón-Gallegos is grateful for EDI, COFAA and SNI fellowship. M. Amezcua-Allieri is grateful for SNI fellowship.
PY - 2008/9
Y1 - 2008/9
N2 - The aim of this work was to evaluate the induction of protoporphyrins IX (PpIX) activity and superoxide anions (SO) in human leukocytes exposed to anthracene (ANT) and benzo(a)pyrene (B(a)P). The leukocyte LC50s for both hydrocarbons and the PpIX accumulation and SO overproduction were measured. The LC50s were 0.35 and 3.23 μM for ANT and B(a)P, respectively. A linear relationship (r = 0.97, p < 0.01) between PpIX and ANT concentration was obtained. The induced accumulation of PpIX was proportional (r = 0.63, p < 0.01) to B(a)P concentration. SO overproduction showed a linear relationship (r = 0.83, p < 0.05) with ANT concentrations. The linear regression analysis of the effect of B(a)P on the superoxide anion overproduction showed a good coefficient (r = 0.97, p < 0.01), showed that ANT and B(a)P exposure induces PpIX accumulation, probably by disruption of the haem biosynthesis. ANT and B(a)P induce SO overproduction, perhaps through a process of redox cycling.
AB - The aim of this work was to evaluate the induction of protoporphyrins IX (PpIX) activity and superoxide anions (SO) in human leukocytes exposed to anthracene (ANT) and benzo(a)pyrene (B(a)P). The leukocyte LC50s for both hydrocarbons and the PpIX accumulation and SO overproduction were measured. The LC50s were 0.35 and 3.23 μM for ANT and B(a)P, respectively. A linear relationship (r = 0.97, p < 0.01) between PpIX and ANT concentration was obtained. The induced accumulation of PpIX was proportional (r = 0.63, p < 0.01) to B(a)P concentration. SO overproduction showed a linear relationship (r = 0.83, p < 0.05) with ANT concentrations. The linear regression analysis of the effect of B(a)P on the superoxide anion overproduction showed a good coefficient (r = 0.97, p < 0.01), showed that ANT and B(a)P exposure induces PpIX accumulation, probably by disruption of the haem biosynthesis. ANT and B(a)P induce SO overproduction, perhaps through a process of redox cycling.
KW - Anthracene
KW - Benzo(a)pyrene
KW - Human leukocytes
KW - Metabolic cell damage
KW - Porphyrins
KW - Superoxide anions
UR - http://www.scopus.com/inward/record.url?scp=47849122010&partnerID=8YFLogxK
U2 - 10.1016/j.etap.2008.05.002
DO - 10.1016/j.etap.2008.05.002
M3 - Artículo
C2 - 21791370
SN - 1382-6689
VL - 26
SP - 237
EP - 240
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
IS - 2
ER -