TY - JOUR
T1 - Candida glabrata survives and replicates in human osteoblasts
AU - Muñoz-Duarte, Ana Rosa
AU - Castrejón-Jiménez, Nayeli Shantal
AU - Baltierra-Uribe, Shantal Lizbeth
AU - Pérez-Rangel, Sofia Judith
AU - Carapia-Minero, Natalee
AU - Castañeda-Sánchez, Jorge Ismael
AU - Luna-Herrera, Julieta
AU - López-Santiago, Rubén
AU - Rodríguez-Tovar, Aída Verónica
AU - García-Pérez, Blanca Estela
N1 - Publisher Copyright:
© FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
PY - 2016/6/1
Y1 - 2016/6/1
N2 - Candida glabrata is an opportunistic pathogen that is considered the second most common cause of candidiasis after Candida albicans Many characteristics of its mechanisms of pathogenicity remain unknown. Recent studies have focused on determining the events that underlie interactions between C. glabrata and immune cells, but the relationship between this yeast and osteoblasts has not been studied in detail. The aim of this study was to determine the mechanisms of interaction between human osteoblasts and C. glabrata, and to identify the roles played by some of the molecules that are produced by these cells in response to infection. We show that C. glabrata adheres to and is internalized by human osteoblasts. Adhesion is independent of opsonization, and internalization depends on the rearrangement of the actin cytoskeleton. We show that C. glabrata survives and replicates in osteoblasts and that this intracellular behavior is related to the level of production of nitric oxide and reactive oxygen species. Opsonized C. glabrata stimulates the production of IL-6, IL-8 and MCP-1 cytokines. Adhesion and internalization of the pathogen and the innate immune response of osteoblasts require viable C. glabrata These results suggest that C. glabrata modulates immunological mechanisms in osteoblasts to survive inside the cell.
AB - Candida glabrata is an opportunistic pathogen that is considered the second most common cause of candidiasis after Candida albicans Many characteristics of its mechanisms of pathogenicity remain unknown. Recent studies have focused on determining the events that underlie interactions between C. glabrata and immune cells, but the relationship between this yeast and osteoblasts has not been studied in detail. The aim of this study was to determine the mechanisms of interaction between human osteoblasts and C. glabrata, and to identify the roles played by some of the molecules that are produced by these cells in response to infection. We show that C. glabrata adheres to and is internalized by human osteoblasts. Adhesion is independent of opsonization, and internalization depends on the rearrangement of the actin cytoskeleton. We show that C. glabrata survives and replicates in osteoblasts and that this intracellular behavior is related to the level of production of nitric oxide and reactive oxygen species. Opsonized C. glabrata stimulates the production of IL-6, IL-8 and MCP-1 cytokines. Adhesion and internalization of the pathogen and the innate immune response of osteoblasts require viable C. glabrata These results suggest that C. glabrata modulates immunological mechanisms in osteoblasts to survive inside the cell.
KW - Candida glabrata
KW - cytokines
KW - cytoskeleton
KW - human osteoblasts
KW - nitric oxide
KW - reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=85021855279&partnerID=8YFLogxK
U2 - 10.1093/femspd/ftw030
DO - 10.1093/femspd/ftw030
M3 - Artículo
SN - 2049-632X
VL - 74
SP - ftw030
JO - Pathogens and disease
JF - Pathogens and disease
IS - 4
ER -