TY - GEN
T1 - Bioimpedance Spectra in Final RT-PCR Products
T2 - 45th Mexican Conference on Biomedical Engineering, CNIB 2022
AU - Padilla García, Karla Lizeth
AU - Gómez López, Modesto
AU - Sánchez Camacho, Jennifer Viridiana
AU - Andrade Torres, Claudia Mariana
AU - Pérez Vielma, Nadia Mabel
AU - González Díaz, César Antonio
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Nature Switzerland AG.
PY - 2023
Y1 - 2023
N2 - Several studies have showed that Deoxyribonucleic Acids (DNA) molecule has a natural electronegative condition and its electrical properties, such as electrical bioimpedance (EBI’s), are strongly associated to DNA concentration. In this work, we propose a simple and preliminary analysis by the use of multifrequency EBI’s measurements to detect amplicons of gene-expression in final Transcriptase Reverse Polymerase Chain Reaction (RT-PCR) products, and the identification of a potential sensitivity threshold point in a specific EBI’s frequency and parameter. Final RT-PCR products for two genes amplification were analyzed by EBIs in a broad frequency range of 100 Hz to 10 MHz. Relative gene expression values were correlated with bioimpedance measurements at every frequency point. The findings suggest a relative gene over-expression threshold sensitivity point in the limit of 0.3, particularly in the phase parameter at 10 MHz.
AB - Several studies have showed that Deoxyribonucleic Acids (DNA) molecule has a natural electronegative condition and its electrical properties, such as electrical bioimpedance (EBI’s), are strongly associated to DNA concentration. In this work, we propose a simple and preliminary analysis by the use of multifrequency EBI’s measurements to detect amplicons of gene-expression in final Transcriptase Reverse Polymerase Chain Reaction (RT-PCR) products, and the identification of a potential sensitivity threshold point in a specific EBI’s frequency and parameter. Final RT-PCR products for two genes amplification were analyzed by EBIs in a broad frequency range of 100 Hz to 10 MHz. Relative gene expression values were correlated with bioimpedance measurements at every frequency point. The findings suggest a relative gene over-expression threshold sensitivity point in the limit of 0.3, particularly in the phase parameter at 10 MHz.
KW - Bioimpedance
KW - DNA
KW - RT-PCR
UR - http://www.scopus.com/inward/record.url?scp=85142692946&partnerID=8YFLogxK
U2 - 10.1007/978-3-031-18256-3_70
DO - 10.1007/978-3-031-18256-3_70
M3 - Contribución a la conferencia
AN - SCOPUS:85142692946
SN - 9783031182556
T3 - IFMBE Proceedings
SP - 665
EP - 673
BT - 45th Mexican Conference on Biomedical Engineering - Proceedings of CNIB 2022
A2 - Trujillo-Romero, Citlalli Jessica
A2 - Gonzalez-Landaeta, Rafael
A2 - Chapa-González, Christian
A2 - Dorantes-Méndez, Guadalupe
A2 - Flores, Dora-Luz
A2 - Flores Cuautle, J.J.Agustin
A2 - Ortiz-Posadas, Martha R.
A2 - Salido Ruiz, Ricardo A.
A2 - Zuñiga-Aguilar, Esmeralda
PB - Springer Science and Business Media Deutschland GmbH
Y2 - 6 October 2022 through 8 October 2022
ER -