TY - JOUR
T1 - Biodegradation of organochlorine pesticides by bacteria grown in microniches of the porous structure of green bean coffee
AU - Barragán-Huerta, B. E.
AU - Costa-Pérez, C.
AU - Peralta-Cruz, J.
AU - Barrera-Cortés, J.
AU - Esparza-García, F.
AU - Rodríguez-Vázquez, R.
N1 - Funding Information:
We would like to thank Ma. Esther Sánchez for taking the microphotographs (ENCB-IPN), Dolores Díaz Cervantes (CINVESTAV-IPN) for her technical help, and Ing. Elvira García (Consejo Mexicano del Café). This study was supported by Consejo Nacional de Ciencia y Tecnología (Fondo Mixto TLAX-2003-C02-12416).
PY - 2007
Y1 - 2007
N2 - In this paper, the authors propose a model for DDT biodegradation by bacteria grown in microniches created in the porous structure of green bean coffee. Five bacteria isolated from coffee beans, identified as Pseudomonas aeruginosa, P. putida, Stenotrophomonas maltophilia, Flavimonas oryzihabitans, and Morganella morganii. P. aeruginosa and F. oryzihabitans, were selected for pesticide degradation. Bacteria were selected according to their ability to grow on mineral media amended with: (a) glucose (10 g l -1 ), (b) peptone (2 g l -1 ), and (c) ground coffee beans (2 g l -1 ). These three media were supplemented with 50 mg l -1 of 1,1,1-trichloro-2,2-bis (4-chlorophenyl) ethane (DDT) and endosulfan. GC/MS analysis demonstrated that the greatest DDT removal was obtained in the medium supplemented with coffee beans, where 1,1-dichloro-2,2′-bis (4-chlorophenyl)ethylene (DDE), 1-chloro-2,2-bis (4-chlorophenyl) ethane (DDMU) and 2,2′-bis (p-chlorophenyl)ethanol (DDOH) were detected. DDMU is a product of the reductive dechlorination of DDE, which in this system could be carried out under the anaerobic conditions in microniches present in the porous structure of the coffee bean. This was supported by scanning electron microscopy. Green bean coffee could be used as a nutrient source and as a support for bacterial growth in pesticide degradation.
AB - In this paper, the authors propose a model for DDT biodegradation by bacteria grown in microniches created in the porous structure of green bean coffee. Five bacteria isolated from coffee beans, identified as Pseudomonas aeruginosa, P. putida, Stenotrophomonas maltophilia, Flavimonas oryzihabitans, and Morganella morganii. P. aeruginosa and F. oryzihabitans, were selected for pesticide degradation. Bacteria were selected according to their ability to grow on mineral media amended with: (a) glucose (10 g l -1 ), (b) peptone (2 g l -1 ), and (c) ground coffee beans (2 g l -1 ). These three media were supplemented with 50 mg l -1 of 1,1,1-trichloro-2,2-bis (4-chlorophenyl) ethane (DDT) and endosulfan. GC/MS analysis demonstrated that the greatest DDT removal was obtained in the medium supplemented with coffee beans, where 1,1-dichloro-2,2′-bis (4-chlorophenyl)ethylene (DDE), 1-chloro-2,2-bis (4-chlorophenyl) ethane (DDMU) and 2,2′-bis (p-chlorophenyl)ethanol (DDOH) were detected. DDMU is a product of the reductive dechlorination of DDE, which in this system could be carried out under the anaerobic conditions in microniches present in the porous structure of the coffee bean. This was supported by scanning electron microscopy. Green bean coffee could be used as a nutrient source and as a support for bacterial growth in pesticide degradation.
KW - Coffee
KW - DDT
KW - Flavimonas
KW - Microniche
KW - Pseudomonas
UR - http://www.scopus.com/inward/record.url?scp=33947508143&partnerID=8YFLogxK
U2 - 10.1016/j.ibiod.2006.11.001
DO - 10.1016/j.ibiod.2006.11.001
M3 - Artículo
SN - 0964-8305
VL - 59
SP - 239
EP - 244
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
IS - 3 SPEC. ISS.
ER -