TY - JOUR
T1 - Biocatalytic chlorination of aromatic hydrocarbons by chloroperoxidase of caldariomyces fumago
AU - Vázquez-Duhalt, Rafael
AU - Ayala, Marcela
AU - Márquez-Rocha, Facundo J.
N1 - Funding Information:
This work was supported by The National Council of Science and Technology of Mexico (CONACYT grant 33611-U) and by The Mexican Oil Institute (grant FIES 98-110-VI).
PY - 2001/11/23
Y1 - 2001/11/23
N2 - Chloroperoxidase from Caldariomyces fumago was able to chlorinate 17 of 20 aromatic hydrocarbons assayed in the presence of hydrogen peroxide and chloride ions. Reaction rates varied from 0.6 min-1 for naphthalene to 758 min-1 for 9-methylanthracene. Mono-, di- and tri-chlorinated compounds were obtained from the chloroperoxidase-mediated reaction on aromatic compounds. Dichloroacenaphthene, trichloroacenaphthene 9,10-dichloroanthracene, chloropyrene, dichloropyrene, dichlorobiphenylene and trichlorobiphenylene were identified by mass spectral analyses as products from acenaphthene, anthracene, pyrene and biophenylene respectively. Polycyclic aromatic hydrocarbons with 5 and 6 aromatic rings were also substrates for the chloroperoxidase reaction. The importance of the microbial chlorination of aromatic pollutants and its potential environmental impact are discussed.
AB - Chloroperoxidase from Caldariomyces fumago was able to chlorinate 17 of 20 aromatic hydrocarbons assayed in the presence of hydrogen peroxide and chloride ions. Reaction rates varied from 0.6 min-1 for naphthalene to 758 min-1 for 9-methylanthracene. Mono-, di- and tri-chlorinated compounds were obtained from the chloroperoxidase-mediated reaction on aromatic compounds. Dichloroacenaphthene, trichloroacenaphthene 9,10-dichloroanthracene, chloropyrene, dichloropyrene, dichlorobiphenylene and trichlorobiphenylene were identified by mass spectral analyses as products from acenaphthene, anthracene, pyrene and biophenylene respectively. Polycyclic aromatic hydrocarbons with 5 and 6 aromatic rings were also substrates for the chloroperoxidase reaction. The importance of the microbial chlorination of aromatic pollutants and its potential environmental impact are discussed.
KW - Enzymatic chlorination
KW - Fungal chloroperoxidase
KW - Polycyclic aromatic hydrocarbons
UR - http://www.scopus.com/inward/record.url?scp=0035940872&partnerID=8YFLogxK
U2 - 10.1016/S0031-9422(01)00326-0
DO - 10.1016/S0031-9422(01)00326-0
M3 - Artículo
C2 - 11684191
AN - SCOPUS:0035940872
SN - 0031-9422
VL - 58
SP - 929
EP - 933
JO - Phytochemistry
JF - Phytochemistry
IS - 6
ER -