TY - JOUR
T1 - Bioassay-guided chemical study of the anti-inflammatory effect of senna villosa (Miller) H.S. Irwin & Barneby (Leguminosae) in TPA-induced ear edema
AU - Del Carmen Susunaga-Notario, Ana
AU - Pérez-Gutiérrez, Salud
AU - Zavala-Sánchez, Miguel Ángel
AU - Almanza-Pérez, Julio Cesar
AU - Gutiérrez-Carrillo, Atilano
AU - Arrieta-Báez, Daniel
AU - López-López, Ana Laura
AU - Román-Ramos, Rubén
AU - Flores-Sáenz, José Luis Eduardo
AU - Alarcón-Aguilar, Francisco Javier
PY - 2014/7
Y1 - 2014/7
N2 - Senna villosa (Miller) is a plant that grows in México. In traditional Mexican medicine, it is used topically to treat skin infections, pustules and eruptions and to heal wounds by scar formation. However, studies of its potential anti-inflammatory effects have not been performed. The aim of the present study was to determine the anti-inflammatory effect of extracts from the leaves of Senna villosa and to perform a bioassay-guided chemical study of the extract with major activity in a model of ear edema induced by 12-O- Tetradecanoylphorbol 13- Acetate (TPA). The results reveal that the chloroform extract from Senna villosa leaves has anti-inflammatory and anti-proliferative properties. Nine fractions were obtained from the bioassay-guided chemical study, including a white precipitate from fractions 2 and 3. Although none of the nine fractions presented anti-inflammatory activity, the white precipitate exhibited pharmacological activity. It was chemically characterized using mass spectrometry and infrared and nuclear magnetic resonance spectroscopy, resulting in a mixture of three aliphatic esters, which were identified as the principal constituents: hexyl tetradecanoate (C20H40O2), heptyl tetradecanoate (C21H42O2) and octyl tetradecanoate (C22H44O2). This research provides, for the first time, evidence of the anti-inflammatory and anti-proliferative properties of compounds isolated from Senna villosa.
AB - Senna villosa (Miller) is a plant that grows in México. In traditional Mexican medicine, it is used topically to treat skin infections, pustules and eruptions and to heal wounds by scar formation. However, studies of its potential anti-inflammatory effects have not been performed. The aim of the present study was to determine the anti-inflammatory effect of extracts from the leaves of Senna villosa and to perform a bioassay-guided chemical study of the extract with major activity in a model of ear edema induced by 12-O- Tetradecanoylphorbol 13- Acetate (TPA). The results reveal that the chloroform extract from Senna villosa leaves has anti-inflammatory and anti-proliferative properties. Nine fractions were obtained from the bioassay-guided chemical study, including a white precipitate from fractions 2 and 3. Although none of the nine fractions presented anti-inflammatory activity, the white precipitate exhibited pharmacological activity. It was chemically characterized using mass spectrometry and infrared and nuclear magnetic resonance spectroscopy, resulting in a mixture of three aliphatic esters, which were identified as the principal constituents: hexyl tetradecanoate (C20H40O2), heptyl tetradecanoate (C21H42O2) and octyl tetradecanoate (C22H44O2). This research provides, for the first time, evidence of the anti-inflammatory and anti-proliferative properties of compounds isolated from Senna villosa.
KW - Anti-inflammatory effect
KW - Anti-proliferative properties
KW - Bioassay-guided chemical study
KW - Senna villosa
UR - http://www.scopus.com/inward/record.url?scp=84904816322&partnerID=8YFLogxK
U2 - 10.3390/molecules190710261
DO - 10.3390/molecules190710261
M3 - Artículo
SN - 1420-3049
VL - 19
SP - 10261
EP - 10278
JO - Molecules
JF - Molecules
IS - 7
ER -