TY - JOUR
T1 - Benzyl amino purine and gibberellic acid coupled to nitrogen-limited stress induce fatty acids, biomass accumulation, and gene expression in scenedesmus obliquus
AU - Correa-Aguado, Hans Christian
AU - Cerrillo-Rojas, Gloria Viviana
AU - Rocha-Uribe, Alejandro
AU - Soria-Guerra, Ruth Elena
AU - Morales-Domínguez, José Francisco
N1 - Publisher Copyright:
© 2021, Tech Science Press. All rights reserved.
PY - 2021
Y1 - 2021
N2 - The need for renewable energy sources makes microalgae an essential feedstock for biofuels production. The molecular aspects and the response to nitrogen (N)-limited conditions with a phytohormone stimulus in micro-algae have been slightly explored. In this work, Scenedesmus obliquus was used as a study model to analyze the effect of benzyl amino purine (BAP) and gibberellic acid (GA) coupled to nitrogen limitation on cell growth, bio-mass and fatty acids. The selected 10-5 M BAP increased the biomass by 1.44-fold, and 10-6 M GA by 1.35-fold. The total lipids also increased by 2.8 and 1.11-fold, respectively. The 10-5 MBAPand10-6 MGAadditionto S. obliquus cultures at different initial nitrogen percentages (N-0, N-25, and N-50) showed a significant increase in cell growth and biomass productivity compared to the unstimulated cultures. BAP N-0 and GA N-0 produced the highest lipid yields with 55% and 50%, respectively. The lipid profile analysis revealed an increase, particularly in C18:1 and C16:0 fatty acids. Gene expression analysis showed an over-expression of acyl carrying protein (ACP), stearoyl-ACP desaturase (SAD), fatty acid acyl-ACP thioesterase (FATA), and diacylglycerol acyltransferase (DGAT) genes, which were mainly induced by nitrogen limitation. Furthermore, BAP and GA produced a significant over-expression on these genes in the N-replete cultures. This study shows that BAP and GA, coupled to N limitation stress, can be used to increase the biomass and lipid production in S. obliquus for sustainable biofuels.
AB - The need for renewable energy sources makes microalgae an essential feedstock for biofuels production. The molecular aspects and the response to nitrogen (N)-limited conditions with a phytohormone stimulus in micro-algae have been slightly explored. In this work, Scenedesmus obliquus was used as a study model to analyze the effect of benzyl amino purine (BAP) and gibberellic acid (GA) coupled to nitrogen limitation on cell growth, bio-mass and fatty acids. The selected 10-5 M BAP increased the biomass by 1.44-fold, and 10-6 M GA by 1.35-fold. The total lipids also increased by 2.8 and 1.11-fold, respectively. The 10-5 MBAPand10-6 MGAadditionto S. obliquus cultures at different initial nitrogen percentages (N-0, N-25, and N-50) showed a significant increase in cell growth and biomass productivity compared to the unstimulated cultures. BAP N-0 and GA N-0 produced the highest lipid yields with 55% and 50%, respectively. The lipid profile analysis revealed an increase, particularly in C18:1 and C16:0 fatty acids. Gene expression analysis showed an over-expression of acyl carrying protein (ACP), stearoyl-ACP desaturase (SAD), fatty acid acyl-ACP thioesterase (FATA), and diacylglycerol acyltransferase (DGAT) genes, which were mainly induced by nitrogen limitation. Furthermore, BAP and GA produced a significant over-expression on these genes in the N-replete cultures. This study shows that BAP and GA, coupled to N limitation stress, can be used to increase the biomass and lipid production in S. obliquus for sustainable biofuels.
KW - Biodiesel
KW - Gene expression
KW - Lipids
KW - Microalgae
KW - Nitrogen deficiency
KW - Phytohormones
UR - http://www.scopus.com/inward/record.url?scp=85102146478&partnerID=8YFLogxK
U2 - 10.32604/phyton.2021.013619
DO - 10.32604/phyton.2021.013619
M3 - Artículo
AN - SCOPUS:85102146478
SN - 0031-9457
VL - 90
SP - 515
EP - 531
JO - Phyton
JF - Phyton
IS - 2
ER -