TY - JOUR
T1 - Bactericide, antioxidant and cytotoxic activities from marine algae of genus laurencia collected in Baja California sur, México
AU - García-Davis, Sara
AU - Murillo-Álvarez, Iván
AU - Muñoz-Ochoa, Mauricio
AU - Carranza-Torres, Edith
AU - Garza-Padrón, Ruth
AU - Morales-Rubio, Eufemia
AU - Viveros-Valdez, Ezequiel
N1 - Publisher Copyright:
© 2018 Sara García-Davis et al.
PY - 2018
Y1 - 2018
N2 - Background and Objective: Marine environment represents countless and diverse resource for new drugs to combat major diseases. Extracts from four Laurencia species (L. johnstonii, L. pacifica, L. gardneri and L. papillosa) from Baja California Sur, México were evaluated for their antioxidant, antibacterial and cytotoxic activity. Methodology: The antioxidants activity of Laurencia sp. were evaluated using the radical scavenging activity in three in vitro radicals: 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2,2'-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and nitric oxide (NO). The antibacterial activity was evaluated by the broth microdilution method to determinate the Minimum Inhibitory Concentrations (MIC) against Staphylococcus aureus, Bacillus subtilis, Enterococcus faecalis, Micrococcus luteus, Pseudomonas aeruginosa and Klebsiella pneumoniae. The cytotoxicity was analyzed on HeLa (cervix adenocarcinoma) and Vero (kidney epithelial) cells, using the reduction of tetrazolium salt WST-1. Results: The seaweed of genus Laurencia demonstrated an overall low activity, with half maximal effective concentration (EC50) values >1.5 mg mLG1. Laurencia pacifica showed the best biocide effects with MIC of 6.25 µg mLG1 against Gram positive bacterial and cytotoxic potential with half inhibitory concentration (IC50) <30 µg mLG1 against Vero and HeLa cells. Conclusion: Some Laurencia species have a great antibacterial and cytotoxic activity which could be considered for future studies.
AB - Background and Objective: Marine environment represents countless and diverse resource for new drugs to combat major diseases. Extracts from four Laurencia species (L. johnstonii, L. pacifica, L. gardneri and L. papillosa) from Baja California Sur, México were evaluated for their antioxidant, antibacterial and cytotoxic activity. Methodology: The antioxidants activity of Laurencia sp. were evaluated using the radical scavenging activity in three in vitro radicals: 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2,2'-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and nitric oxide (NO). The antibacterial activity was evaluated by the broth microdilution method to determinate the Minimum Inhibitory Concentrations (MIC) against Staphylococcus aureus, Bacillus subtilis, Enterococcus faecalis, Micrococcus luteus, Pseudomonas aeruginosa and Klebsiella pneumoniae. The cytotoxicity was analyzed on HeLa (cervix adenocarcinoma) and Vero (kidney epithelial) cells, using the reduction of tetrazolium salt WST-1. Results: The seaweed of genus Laurencia demonstrated an overall low activity, with half maximal effective concentration (EC50) values >1.5 mg mLG1. Laurencia pacifica showed the best biocide effects with MIC of 6.25 µg mLG1 against Gram positive bacterial and cytotoxic potential with half inhibitory concentration (IC50) <30 µg mLG1 against Vero and HeLa cells. Conclusion: Some Laurencia species have a great antibacterial and cytotoxic activity which could be considered for future studies.
KW - Algal extract
KW - Antioxidant activity
KW - Bioactive metabolites
KW - Bioprospection
KW - Cytotoxic activity
KW - Laurencia
KW - Marine antibacterial
KW - Seaweeds
UR - http://www.scopus.com/inward/record.url?scp=85044967676&partnerID=8YFLogxK
U2 - 10.3923/ijp.2018.391.396
DO - 10.3923/ijp.2018.391.396
M3 - Artículo
AN - SCOPUS:85044967676
SN - 1811-7775
VL - 14
SP - 391
EP - 396
JO - International Journal of Pharmacology
JF - International Journal of Pharmacology
IS - 3
ER -