TY - JOUR
T1 - Bacterial community structure in the rhizosphere of three cactus species from semi-arid highlands in central Mexico
AU - Aguirre-Garrido, J. Félix
AU - Montiel-Lugo, Daniel
AU - Hernández-Rodríguez, César
AU - Torres-Cortes, Gloria
AU - Millán, Vicenta
AU - Toro, Nicolás
AU - Martínez-Abarca, Francisco
AU - Ramírez-Saad, Hugo C.
N1 - Funding Information:
Acknowledgments This work received financial support from Foundation BBVA, trough project BIOCON 04-084. F.A.G. received fellowships from CONACyT and COMECyT (Mexico), for PhD studies.
PY - 2012/5
Y1 - 2012/5
N2 - The nature reserve of Tehuacan-Cuicatlan in central Mexico is known for its diversity and endemism mainly in cactus plants. Although the xerophytic flora is reasonably documented, the bacterial communities associated with these species have been largely neglected. We assessed the diversity and composition of bacterial communities in bulk (non-rhizospheric) soil and the rhizosphere of three cactus plant species: Mammillaria carnea, Opuntia pilifera and Stenocereus stellatus, approached using cultivation and molecular techniques, considering the possible effect of dry and rainy seasons. Cultivationdependent methods were focused on putative N 2-fixers and heterotrophic aerobic bacteria, in the two media tested the values obtained for dry season samples grouped together regardless of the sample type (rhizospheric or non-rhizospheric), these groups also included the non-rhizospheric sample for rainy season, on each medium. These CFU values were smaller and significantly different from those obtained on rhizospheric samples from rainy season. Genera composition among isolates of the rhizospheric samples was very similar for each season, the most abundant taxa being a-Proteobacteria, Actinobacteria and Firmicutes. Interestingly, the genus Ochrobactrum was highly represented among rhizospheric samples, when cultured in N-free medium. The structure of the bacterial communities was approached with molecular techniques targeting partial 16S rRNA sequences such as denaturing gradient gel electrophoresis and serial analysis of ribosomal sequence tags. Under these approaches, the most represented bacterial phyla were Actinobacteria, Proteobacteria and Acidobacteria. The first two were also highly represented when using isolation techniques.
AB - The nature reserve of Tehuacan-Cuicatlan in central Mexico is known for its diversity and endemism mainly in cactus plants. Although the xerophytic flora is reasonably documented, the bacterial communities associated with these species have been largely neglected. We assessed the diversity and composition of bacterial communities in bulk (non-rhizospheric) soil and the rhizosphere of three cactus plant species: Mammillaria carnea, Opuntia pilifera and Stenocereus stellatus, approached using cultivation and molecular techniques, considering the possible effect of dry and rainy seasons. Cultivationdependent methods were focused on putative N 2-fixers and heterotrophic aerobic bacteria, in the two media tested the values obtained for dry season samples grouped together regardless of the sample type (rhizospheric or non-rhizospheric), these groups also included the non-rhizospheric sample for rainy season, on each medium. These CFU values were smaller and significantly different from those obtained on rhizospheric samples from rainy season. Genera composition among isolates of the rhizospheric samples was very similar for each season, the most abundant taxa being a-Proteobacteria, Actinobacteria and Firmicutes. Interestingly, the genus Ochrobactrum was highly represented among rhizospheric samples, when cultured in N-free medium. The structure of the bacterial communities was approached with molecular techniques targeting partial 16S rRNA sequences such as denaturing gradient gel electrophoresis and serial analysis of ribosomal sequence tags. Under these approaches, the most represented bacterial phyla were Actinobacteria, Proteobacteria and Acidobacteria. The first two were also highly represented when using isolation techniques.
KW - Cactus
KW - DGGE
KW - Mammillaria
KW - Metagenomic rhizosphere and NifH
KW - Opuntia
KW - Rhizosphere
KW - SARST
KW - Stenocereus
UR - http://www.scopus.com/inward/record.url?scp=84862687738&partnerID=8YFLogxK
U2 - 10.1007/s10482-012-9705-3
DO - 10.1007/s10482-012-9705-3
M3 - Artículo
C2 - 22307841
SN - 0003-6072
VL - 101
SP - 891
EP - 904
JO - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
JF - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
IS - 4
ER -