TY - JOUR
T1 - Authors information
AU - Ramos-García, Víctor Hugo
AU - Villota-Salazar, Nubia Andrea
AU - González-Prieto, Juan Manuel
AU - Cortés-Espinosa, Diana V.
N1 - Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature B.V.
PY - 2022/4
Y1 - 2022/4
N2 - Fungal phytopathogens require different skills to infect plants and complete their lifecycle. Some proteins in fungi are essential for pathogenesis and their expression is regulated by epigenetic mechanisms via chromatin-remodeling. Macrophomina phaseolina is an important phytopathogenic fungus that leads to considerable losses of different crops, especially during drought conditions. Some biological features of the fungus have been described. However, the epigenetics mechanisms involved in the development and virulence of M. phaseolina have not been fully studied. In this work, chemical inhibition was used to evaluate the role of histone deacetylases (HDACs) in the biology of M. phaseolina. The effect of two histone deacetylase inhibitors (iHDAC), valproic acid (VPA) and sodium butyrate (SBT), was analyzed. The results showed that the treated fungus presented a decrease in microsclerotia diameter, aerial mycelia production, vegetative growth, and cell pigmentation. In addition, VPA and SBT also affected the ability of the fungus to grow on complex carbon sources and virulence in the bean variety, BAT 477. Thus, it can be concluded that the alteration of histone deacetylation by VPA and SBT affects M. phaseolina growth, morphology, and virulence. Graphical abstract: [Figure not available: see fulltext.]
AB - Fungal phytopathogens require different skills to infect plants and complete their lifecycle. Some proteins in fungi are essential for pathogenesis and their expression is regulated by epigenetic mechanisms via chromatin-remodeling. Macrophomina phaseolina is an important phytopathogenic fungus that leads to considerable losses of different crops, especially during drought conditions. Some biological features of the fungus have been described. However, the epigenetics mechanisms involved in the development and virulence of M. phaseolina have not been fully studied. In this work, chemical inhibition was used to evaluate the role of histone deacetylases (HDACs) in the biology of M. phaseolina. The effect of two histone deacetylase inhibitors (iHDAC), valproic acid (VPA) and sodium butyrate (SBT), was analyzed. The results showed that the treated fungus presented a decrease in microsclerotia diameter, aerial mycelia production, vegetative growth, and cell pigmentation. In addition, VPA and SBT also affected the ability of the fungus to grow on complex carbon sources and virulence in the bean variety, BAT 477. Thus, it can be concluded that the alteration of histone deacetylation by VPA and SBT affects M. phaseolina growth, morphology, and virulence. Graphical abstract: [Figure not available: see fulltext.]
KW - Epigenetic
KW - Fungal phytopathogens
KW - Histone deacetylase inhibitors
KW - Macrophomina phaseolina
KW - Sodium butyrate
KW - Valproic acid
UR - http://www.scopus.com/inward/record.url?scp=85125553534&partnerID=8YFLogxK
U2 - 10.1007/s11274-022-03249-w
DO - 10.1007/s11274-022-03249-w
M3 - Artículo
C2 - 35226232
AN - SCOPUS:85125553534
SN - 0959-3993
VL - 38
JO - World Journal of Microbiology and Biotechnology
JF - World Journal of Microbiology and Biotechnology
IS - 4
M1 - 63
ER -