TY - JOUR
T1 - Asociación de células NKT con la expresión del gen CTSL en población pediátrica Mexicana con diabetes tipo 1 de reciente diagnostic
AU - Gómez-Díaz, Rita Angélica
AU - Medina-Santillán, Roberto
AU - Castro-Magdonel, Blanca Elena
AU - Bekker-Méndez, Carolina
AU - Gómez-Zamudio, Jaime
AU - Nishimura-Meguro, Elisa
AU - Garrido-Magaña, Eulalia
AU - Lizárraga-Paulin, Lorena
AU - Aguilar-Herrera, Blanca E.
AU - Valladares-Salgado, Adán
AU - Cruz, Miguel
AU - Mondragón-González, Rafael
AU - Ortiz-Navarrete, Vianney
AU - Wacher, Niels H.
N1 - Publisher Copyright:
© 2016,Academia Nacional de Medicina. All rights reserved.
PY - 2016/10
Y1 - 2016/10
N2 - Objective: To compare the level of expression of the gene CTSL and its correlation with NKT cells in patients with recent-onset type 1 diabetes (T1D),their siblings,and healthy controls. Methods: Analytical cross-sectional design. Patients with T1D < 3 months evolution,their siblings,and healthy controls were included. Percentages and absolute numbers of NKT cells were measured with expression of the CTSL gene. Results: 124 subjects: with T1D (n = 48),siblings (n = 44) and controls (n = 32) were included. HbA1c was greater and C-peptide lower in T1D than the other groups and sibling age was higher (p < 0.001). There were no differences in NKT cells between T1D (0.176 ± 0.202) and controls (0.118 ± 0.133),but the percentage was higher in siblings (0.246 ± 0.188; p = 0.002). Lower level of expression of the CTSL gene associated with both absolute number (r: 0.4607; 95% CI: –0.08425 to –0.7935; p = 0.043) and percentage of NKT cells (r: 0.4540; 95% CI: –0.0927 to –0.7903; p = 0.045) in the T1D group. Conclusions: Patients with T1D have lower percentage and absolute number of NKT cells compared to their siblings. NKT cells absolute numbers are correlated with the expression of CTSL in T1D patients.
AB - Objective: To compare the level of expression of the gene CTSL and its correlation with NKT cells in patients with recent-onset type 1 diabetes (T1D),their siblings,and healthy controls. Methods: Analytical cross-sectional design. Patients with T1D < 3 months evolution,their siblings,and healthy controls were included. Percentages and absolute numbers of NKT cells were measured with expression of the CTSL gene. Results: 124 subjects: with T1D (n = 48),siblings (n = 44) and controls (n = 32) were included. HbA1c was greater and C-peptide lower in T1D than the other groups and sibling age was higher (p < 0.001). There were no differences in NKT cells between T1D (0.176 ± 0.202) and controls (0.118 ± 0.133),but the percentage was higher in siblings (0.246 ± 0.188; p = 0.002). Lower level of expression of the CTSL gene associated with both absolute number (r: 0.4607; 95% CI: –0.08425 to –0.7935; p = 0.043) and percentage of NKT cells (r: 0.4540; 95% CI: –0.0927 to –0.7903; p = 0.045) in the T1D group. Conclusions: Patients with T1D have lower percentage and absolute number of NKT cells compared to their siblings. NKT cells absolute numbers are correlated with the expression of CTSL in T1D patients.
KW - Cathepsin-L
KW - Healthy control
KW - NKT cell
KW - Sibling
KW - Type 1 diabetes
UR - http://www.scopus.com/inward/record.url?scp=84996554019&partnerID=8YFLogxK
M3 - Artículo
SN - 0016-3813
VL - 152
SP - 14
EP - 21
JO - Gaceta Medica de Mexico
JF - Gaceta Medica de Mexico
ER -