TY - JOUR
T1 - Antimutagenic activity of phenolic compounds, oligosaccharides and quinolizidinic alkaloids from Lupinus campestris seeds
AU - Jiménez Martínez, C.
AU - Loarca-Piña, G.
AU - Dávila Ortíz, G.
N1 - Funding Information:
The authors thank the Instituto Politécnico Nacional (IPN) and Consejo Nacional de Ciencia y Tecnología (CONACyT) through 33995 Project for financial support. Cristian Jiménez Martinez acknowledges a study grant from CONACyT. The authors thank Paul Kuzmicky, the University of California at Davis, for comments on the manuscript.
PY - 2003/10
Y1 - 2003/10
N2 - There are some foods that contain mutagenic or carcinogenic agents, some of which occur naturally and others that may be formed during preparation or cooking. Several foods, such as legumes, also contain natural antimutagens and/or anticarcinogens. Lupine is one such legume that contains high amounts of protein (40%) and oils (14%). About 90 species of lupine have been reported throughout Mexico. However, the use of this crop as a source of food has been limited by the presence of antinutritional agents such as phenolic compounds (PC), carbohydrates (CH) and quinolizidinic alkaloids (Qas). It has also been suggested that consuming these compounds can affect human health and may even reduce the risk of disease. The objective of this work was to determine the effect of PC, CH and Qas, isolated and quantified from Lupinus campestris on the mutagenicity of 1-nitropyrene (1-NP) as a model mutagen and we used the Salmonella typhimurium tester strain YG1024 by the Kado microsuspension method. The results indicate that L. Campestris seeds have 11 mg (+) catechin equivalent g-1 seed coat; 120.3 mg g-1 seeds and 2.13mg g-] seeds of PC, CH and Qas, respectively. 1-NP mutagenicity was inhibited by 86% for PC, 76% for CH and 75% for Qas at concentrations of 200, 512 and 13.6 μg/tube, respectively.
AB - There are some foods that contain mutagenic or carcinogenic agents, some of which occur naturally and others that may be formed during preparation or cooking. Several foods, such as legumes, also contain natural antimutagens and/or anticarcinogens. Lupine is one such legume that contains high amounts of protein (40%) and oils (14%). About 90 species of lupine have been reported throughout Mexico. However, the use of this crop as a source of food has been limited by the presence of antinutritional agents such as phenolic compounds (PC), carbohydrates (CH) and quinolizidinic alkaloids (Qas). It has also been suggested that consuming these compounds can affect human health and may even reduce the risk of disease. The objective of this work was to determine the effect of PC, CH and Qas, isolated and quantified from Lupinus campestris on the mutagenicity of 1-nitropyrene (1-NP) as a model mutagen and we used the Salmonella typhimurium tester strain YG1024 by the Kado microsuspension method. The results indicate that L. Campestris seeds have 11 mg (+) catechin equivalent g-1 seed coat; 120.3 mg g-1 seeds and 2.13mg g-] seeds of PC, CH and Qas, respectively. 1-NP mutagenicity was inhibited by 86% for PC, 76% for CH and 75% for Qas at concentrations of 200, 512 and 13.6 μg/tube, respectively.
KW - 1-nitropyrene
KW - Antimutagenicity
KW - Carbohydrates
KW - Microsuspension assay
KW - Mutagenicity
KW - Phenolic compounds
KW - Quinolizidinic alkaloids
UR - http://www.scopus.com/inward/record.url?scp=0242659095&partnerID=8YFLogxK
U2 - 10.1080/02652030310001605998
DO - 10.1080/02652030310001605998
M3 - Artículo
C2 - 14594678
SN - 0265-203X
VL - 20
SP - 940
EP - 948
JO - Food Additives and Contaminants
JF - Food Additives and Contaminants
IS - 10
ER -