TY - JOUR
T1 - Antibacterial activity in the hemolymph of the catarina scallop Argopecten ventricosus
AU - Luna-González, Antonio
AU - Maeda-Martínez, Alfonso
AU - Campa-Córdova, Ángel
AU - Orduña-Rojas, Javier
PY - 2007/4
Y1 - 2007/4
N2 - We conducted a search for antibacterial peptide like activity in hemolymph of Argopecten ventricosus. Pre-purification of peptides was done by reverse phase HPLC. Hemolymph acidic supernatant was loaded into a column packed with C18 matrix. Stepwise elutions were performed with 5, 50, 80, and 100 % acetonitrile (ACN) in 0.05 % trifluoroacetic acid (TFA) over 550 min at a flow rate of 0.2 ml/min. Absorbance was monitored at 280 nm. Eluted fractions were concentrated under vacuum. Vibrio alginolyticus 138-2 was used as a model to test fractions. Growth inhibition zone were observed at 5 and 50 % ACN. This is the first report of antimicrobial peptide-like activity in hemolymph of a pectinid species.
AB - We conducted a search for antibacterial peptide like activity in hemolymph of Argopecten ventricosus. Pre-purification of peptides was done by reverse phase HPLC. Hemolymph acidic supernatant was loaded into a column packed with C18 matrix. Stepwise elutions were performed with 5, 50, 80, and 100 % acetonitrile (ACN) in 0.05 % trifluoroacetic acid (TFA) over 550 min at a flow rate of 0.2 ml/min. Absorbance was monitored at 280 nm. Eluted fractions were concentrated under vacuum. Vibrio alginolyticus 138-2 was used as a model to test fractions. Growth inhibition zone were observed at 5 and 50 % ACN. This is the first report of antimicrobial peptide-like activity in hemolymph of a pectinid species.
KW - Antimicrobial peptide
KW - Argopecten ventricosus
KW - Hemolymph
KW - Immunity
KW - Vibrio alginolyticus
UR - http://www.scopus.com/inward/record.url?scp=84869106615&partnerID=8YFLogxK
M3 - Artículo
SN - 0188-8897
VL - 17
SP - 87
EP - 89
JO - Hidrobiologica
JF - Hidrobiologica
IS - 1
ER -