TY - JOUR
T1 - Anti-inflammatory and anti-allodynic activities in mice of the essential oil and desmethylisoencecalin from Zinnia grandiflora Nuttall
AU - Reyes-Pérez, Valeria
AU - Granados-Soto, Vinicio
AU - Linares, Edelmira
AU - Bye, Robert
AU - Mata, Rachel
AU - Deciga-Campos, Myrna
N1 - Publisher Copyright:
© 2022 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2022
Y1 - 2022
N2 - Zinnia grandiflora (Asteraceae) is used to alleviate several painful disorders. The effects of an essential oil prepared by hydrodistillation from Z. grandiflora (EOZ) and its major compound on neuropathic pain, allodynia, and inflammation in mice were assessed. Headspace-solid phase microextraction analysis of an aqueous extract was also carried out. GC-MS analyses of all volatiles revealed that germacrene D (13.24%) and desmethylisoencecalin (DME) (13.24%) were the most abundant compounds. EOZ was effective in reducing inflammation and allodynia in mice. EOZ (300 mg/paw) reverted tactile allodynia more effectively than gabapentin (100 μg/paw). DME (0.1‒10 µg/paw) responses in the carrageenan model were concentration-dependent; the maximum effect was observed at 10 µg/paw, like that of diclofenac (30 μg/paw). In the spinal nerve ligation model, DME (0.1‒10 μg/paw) exerted a concentration-dependent anti-allodynic action; its effect was better than that of gabapentin. A method to quantify zinagrandinolide E in the crude drug was developed and validated.
AB - Zinnia grandiflora (Asteraceae) is used to alleviate several painful disorders. The effects of an essential oil prepared by hydrodistillation from Z. grandiflora (EOZ) and its major compound on neuropathic pain, allodynia, and inflammation in mice were assessed. Headspace-solid phase microextraction analysis of an aqueous extract was also carried out. GC-MS analyses of all volatiles revealed that germacrene D (13.24%) and desmethylisoencecalin (DME) (13.24%) were the most abundant compounds. EOZ was effective in reducing inflammation and allodynia in mice. EOZ (300 mg/paw) reverted tactile allodynia more effectively than gabapentin (100 μg/paw). DME (0.1‒10 µg/paw) responses in the carrageenan model were concentration-dependent; the maximum effect was observed at 10 µg/paw, like that of diclofenac (30 μg/paw). In the spinal nerve ligation model, DME (0.1‒10 μg/paw) exerted a concentration-dependent anti-allodynic action; its effect was better than that of gabapentin. A method to quantify zinagrandinolide E in the crude drug was developed and validated.
KW - Zinnia grandiflora
KW - allodynia
KW - desmethylisoencecalin
KW - essential oil
KW - inflammation
KW - neuropathic pain
UR - http://www.scopus.com/inward/record.url?scp=85139071338&partnerID=8YFLogxK
U2 - 10.1080/10412905.2022.2118880
DO - 10.1080/10412905.2022.2118880
M3 - Artículo
AN - SCOPUS:85139071338
SN - 1041-2905
VL - 34
SP - 490
EP - 501
JO - Journal of Essential Oil Research
JF - Journal of Essential Oil Research
IS - 6
ER -