TY - JOUR
T1 - Anti-inflammatory activity of different agave plants and the compound Cantalasaponin-1
AU - Monterrosas-Brisson, Nayeli
AU - Arenas Ocampo, Martha L.
AU - Jiménez-Ferrer, Enrique
AU - Jiménez-Aparicio, Antonio R.
AU - Zamilpa, Alejandro
AU - Gonzalez-Cortazar, Manases
AU - Tortoriello, Jaime
AU - Herrera-Ruiz, Maribel
PY - 2013/7
Y1 - 2013/7
N2 - Species of the agave genus, such as Agave tequilana, Agave angustifolia and Agave americana are used in Mexican traditional medicine to treat inflammation-associated conditions. These plants' leaves contain saponin compounds which show anti-inflammatory properties in different models. The goal of this investigation was to evaluate the anti-inflammatory capacity of these plants, identify which is the most active, and isolate the active compound by a bio-directed fractionation using the ear edema induced in mice with 12-O-tetradecanoylphorbol-13-acetate (TPA) technique. A dose of 6 mg/ear of acetone extract from the three agave species induced anti-inflammatory effects, however, the one from A. americana proved to be the most active. Different fractions of this species showed biological activity. Finally the F5 fraction at 2.0 mg/ear induced an inhibition of 85.6%. We identified one compound in this fraction as (25R)-5α-spirostan-3β,6α,23α-triol- 3,6-di-O-β-D-glucopyranoside (cantalasaponin-1) through 1H- and 13C-NMR spectral analysis and two dimensional experiments like DEPT NMR, COSY, HSQC and HMBC. This steroidal glycoside showed a dose dependent effect of up to 90% of ear edema inhibition at the highest dose of 1.5 mg/ear.
AB - Species of the agave genus, such as Agave tequilana, Agave angustifolia and Agave americana are used in Mexican traditional medicine to treat inflammation-associated conditions. These plants' leaves contain saponin compounds which show anti-inflammatory properties in different models. The goal of this investigation was to evaluate the anti-inflammatory capacity of these plants, identify which is the most active, and isolate the active compound by a bio-directed fractionation using the ear edema induced in mice with 12-O-tetradecanoylphorbol-13-acetate (TPA) technique. A dose of 6 mg/ear of acetone extract from the three agave species induced anti-inflammatory effects, however, the one from A. americana proved to be the most active. Different fractions of this species showed biological activity. Finally the F5 fraction at 2.0 mg/ear induced an inhibition of 85.6%. We identified one compound in this fraction as (25R)-5α-spirostan-3β,6α,23α-triol- 3,6-di-O-β-D-glucopyranoside (cantalasaponin-1) through 1H- and 13C-NMR spectral analysis and two dimensional experiments like DEPT NMR, COSY, HSQC and HMBC. This steroidal glycoside showed a dose dependent effect of up to 90% of ear edema inhibition at the highest dose of 1.5 mg/ear.
KW - A. americana
KW - A. angustifolia Haw
KW - A. tequilana Weber
KW - Agavaceae
KW - Cantalasaponin-1
KW - Inflammation
UR - http://www.scopus.com/inward/record.url?scp=84880795248&partnerID=8YFLogxK
U2 - 10.3390/molecules18078136
DO - 10.3390/molecules18078136
M3 - Artículo
C2 - 23846754
SN - 1420-3049
VL - 18
SP - 8136
EP - 8146
JO - Molecules
JF - Molecules
IS - 7
ER -