TY - JOUR
T1 - Anabolic androgenic steroids induce micronuclei in bucca mucosa cells of bodybuilders
AU - Torres-Bugarín, O.
AU - Covarrubias-Bugarín, R.
AU - Zamora-Perez, A. L.
AU - Torres-Mendoza, B. M.G.
AU - García-Ulloa, M.
AU - Martínez-Sandoval, F. G.
PY - 2007/9
Y1 - 2007/9
N2 - Objective: To evaluate genotoxicity of anabolic androgenic steroids (AAS) in male bodybuilders by a micronucleus assay in buccal mucosa cells. Methods: 11 male bodybuilders volunteered to participate in this study and two groups were formed: group 1 (n = 6), without AAS consumption and group 2 (n = 5), with AAS consumption. A sample of buccal epithelium was taken from each participant once a week for 6 weeks. Samples were fixed, stained and analysed by a light microscope, and 2000 cells were counted from each slide. Results are expressed as micronucleated cells (MNC) per 1000 cells and were analysed by the Mann-Whitney U test and Wilcoxon's test. Results: A marked increased in MNC was seen in bodybuilders with AAS consumption compared with those without AAS consumption (mean (SD) 4.1 (2.4) MNC/1000 cells vs 0.4 (0.4) MNC/1000 cells, respectively; p<0.004). Intragroup comparisons showed no differences in the MNC frequencies during the sampling time in group 1, whereas the MNC frequency in group 2 varied significantly, reaching the highest MNC frequencies in the third and fourth week of sampling (5.9 (2.4) MNC/1000 cells; 5.8 (1.8) MNC/1000 cells, respectively); frequency in the first sampled week was 1.1 (0.1) MNC/1000 cells. Significant differences in all sampled weeks were found between the two groups. Conclusion: AAS consumption increased the frequency of MNC from buccal mucosa in bodybuilders.
AB - Objective: To evaluate genotoxicity of anabolic androgenic steroids (AAS) in male bodybuilders by a micronucleus assay in buccal mucosa cells. Methods: 11 male bodybuilders volunteered to participate in this study and two groups were formed: group 1 (n = 6), without AAS consumption and group 2 (n = 5), with AAS consumption. A sample of buccal epithelium was taken from each participant once a week for 6 weeks. Samples were fixed, stained and analysed by a light microscope, and 2000 cells were counted from each slide. Results are expressed as micronucleated cells (MNC) per 1000 cells and were analysed by the Mann-Whitney U test and Wilcoxon's test. Results: A marked increased in MNC was seen in bodybuilders with AAS consumption compared with those without AAS consumption (mean (SD) 4.1 (2.4) MNC/1000 cells vs 0.4 (0.4) MNC/1000 cells, respectively; p<0.004). Intragroup comparisons showed no differences in the MNC frequencies during the sampling time in group 1, whereas the MNC frequency in group 2 varied significantly, reaching the highest MNC frequencies in the third and fourth week of sampling (5.9 (2.4) MNC/1000 cells; 5.8 (1.8) MNC/1000 cells, respectively); frequency in the first sampled week was 1.1 (0.1) MNC/1000 cells. Significant differences in all sampled weeks were found between the two groups. Conclusion: AAS consumption increased the frequency of MNC from buccal mucosa in bodybuilders.
UR - http://www.scopus.com/inward/record.url?scp=34548787730&partnerID=8YFLogxK
U2 - 10.1136/bjsm.2006.032474
DO - 10.1136/bjsm.2006.032474
M3 - Artículo
SN - 0306-3674
VL - 41
SP - 592
EP - 596
JO - British Journal of Sports Medicine
JF - British Journal of Sports Medicine
IS - 9
ER -