TY - JOUR
T1 - An efficient method for miRNA detection and localization in crop plants
AU - de Fátima Rosas-Cárdenas, Flor
AU - Escobar-Guzmán, Rocío
AU - Cruz-Hernández, Andrés
AU - Marsch-Martínez, Nayelli
AU - de Folter, Stefan
N1 - Publisher Copyright:
© 2015 Rosas-Cárdenas, Escobar-Guzmán, Cruz-Hernández, Marsch-Martínez and de Folter.
PY - 2015/3/3
Y1 - 2015/3/3
N2 - microRNAs are a class of non-coding small RNAs (sRNAs) that are important regulators of gene expression at the post-transcriptional level by mRNA cleavage or translation inhibition. Another class of sRNAs are siRNAs, which also regulate gene expression but by causing DNA methylation. This epigenetic regulatory role has been observed for some miRNAs as well. The use of sRNAs allows the development of biotechnological applications in plants. To develop these types of applications, and to better understand the natural roles they play, it is important to be able to detect and to localize these sRNAs at the plant tissue level. Sometimes, in crop plants this can be challenging. Therefore, we developed a tissue printing hybridization protocol for easy and efficient detection of sRNAs and demonstrate this by the analysis of the spatio-temporal expression patterns of the miRNAs miR159 and miR164 in fruits of various crop plants. Moreover, we show the possibility to also detect the expression of miRNAs in fruit juice using a dot blot hybridization approach.
AB - microRNAs are a class of non-coding small RNAs (sRNAs) that are important regulators of gene expression at the post-transcriptional level by mRNA cleavage or translation inhibition. Another class of sRNAs are siRNAs, which also regulate gene expression but by causing DNA methylation. This epigenetic regulatory role has been observed for some miRNAs as well. The use of sRNAs allows the development of biotechnological applications in plants. To develop these types of applications, and to better understand the natural roles they play, it is important to be able to detect and to localize these sRNAs at the plant tissue level. Sometimes, in crop plants this can be challenging. Therefore, we developed a tissue printing hybridization protocol for easy and efficient detection of sRNAs and demonstrate this by the analysis of the spatio-temporal expression patterns of the miRNAs miR159 and miR164 in fruits of various crop plants. Moreover, we show the possibility to also detect the expression of miRNAs in fruit juice using a dot blot hybridization approach.
KW - Fruits
KW - Hybridization
KW - MiR164
KW - MiRNA detection
KW - Tissue-printing
UR - http://www.scopus.com/inward/record.url?scp=84925114409&partnerID=8YFLogxK
U2 - 10.3389/fpls.2015.00099
DO - 10.3389/fpls.2015.00099
M3 - Artículo
C2 - 25784917
SN - 1664-462X
VL - 6
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
IS - MAR
M1 - 99
ER -