TY - JOUR
T1 - Aluminum-induced oxidative stress in lymphocytes of common carp (Cyprinus carpio)
AU - García-Medina, Sandra
AU - Razo-Estrada, Amparo Celene
AU - Gómez-Oliván, Leobardo Manuel
AU - Amaya-Chávez, Araceli
AU - Madrigal-Bujaidar, Eduardo
AU - Galar-Martínez, Marcela
N1 - Funding Information:
Acknowledgments This study was made possible through support from the National Science and Technology Council (CONACyT, Project 57321) as well as the Research and Postgraduate Division of the National Polytechnic Institute, Mexico (SIP-IPN, Projects 20060186 and 200704594).
PY - 2010/12
Y1 - 2010/12
N2 - Several studies of fish have shown that aluminum may induce hypoxia, hypercapnia, metabolic acidosis, and respiratory failure. In lymphocytes, morphologic abnormalities and reduced immune activity have been observed. Nevertheless, there is little data on oxidative stress and such data are essential in order to identify its mechanism of action. The common carp Cyprinus carpio, an omnivorous fish commonly used in commercial aquaculture, has been proposed as a test organism in toxicologic assays due to its economic importance and wide geographic distribution. The aim of this work was to evaluate Al-induced oxidative stress in lymphocytes of the common carp C. carpio. Specimens were exposed to three different concentrations of Al (0. 05, 120, and 239 mg/l) in a static exposure system for 96 h. At the end of the exposure period, blood was collected and lymphocytes were separated. Lipid peroxidation, oxidized protein content and the activity of superoxide dismutase, catalase, and glutathione peroxidase were measured. Results show that the tested Al concentrations modified the activity of antioxidant enzymes and elicited higher levels of lipid peroxidation and oxidized proteins. The degree of damage induced was concentration and tissue dependent.
AB - Several studies of fish have shown that aluminum may induce hypoxia, hypercapnia, metabolic acidosis, and respiratory failure. In lymphocytes, morphologic abnormalities and reduced immune activity have been observed. Nevertheless, there is little data on oxidative stress and such data are essential in order to identify its mechanism of action. The common carp Cyprinus carpio, an omnivorous fish commonly used in commercial aquaculture, has been proposed as a test organism in toxicologic assays due to its economic importance and wide geographic distribution. The aim of this work was to evaluate Al-induced oxidative stress in lymphocytes of the common carp C. carpio. Specimens were exposed to three different concentrations of Al (0. 05, 120, and 239 mg/l) in a static exposure system for 96 h. At the end of the exposure period, blood was collected and lymphocytes were separated. Lipid peroxidation, oxidized protein content and the activity of superoxide dismutase, catalase, and glutathione peroxidase were measured. Results show that the tested Al concentrations modified the activity of antioxidant enzymes and elicited higher levels of lipid peroxidation and oxidized proteins. The degree of damage induced was concentration and tissue dependent.
KW - Aluminum
KW - Cyprinus carpio
KW - Lymphocytes
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=78149500461&partnerID=8YFLogxK
U2 - 10.1007/s10695-009-9363-1
DO - 10.1007/s10695-009-9363-1
M3 - Artículo
C2 - 19866366
SN - 0920-1742
VL - 36
SP - 875
EP - 882
JO - Fish Physiology and Biochemistry
JF - Fish Physiology and Biochemistry
IS - 4
ER -