TY - JOUR
T1 - Alternatives to classic solvents for the isolation of bioactive compounds from Chrysochromulina rotalis
AU - González-Cardoso, M. A.
AU - Cerón-García, M. C.
AU - Navarro-López, E.
AU - Molina-Miras, A.
AU - Sánchez-Mirón, A.
AU - Contreras-Gómez, A.
AU - García-Camacho, F.
N1 - Publisher Copyright:
© 2023 The Author(s)
PY - 2023/7
Y1 - 2023/7
N2 - This paper demonstrates a sequential partitioning method for isolating bioactive compounds from Chrysochromulina rotalis using a polarity gradient, replacing classic and hazardous solvents with greener alternatives. Seventeen solvents were evaluated based on their Hansen solubility parameters and for having a similar polarity to the solvents they would replace, four of which were selected as substitutes in the classic fractionation process. Considering the fatty acid and carotenoid recovery yields obtained for each of the solvents, it has been proposed to replace hexane (HEX), toluene (TOL), dichloromethane (DCM) and n-butanol (BUT) with cyclohexane, chlorobenzene, isobutyl acetate and isoamyl alcohol, respectively. In addition, cytotoxic activity was observed when the TOL and DCM solvent extracts were tested against tumour cell lines, demonstrating the antiproliferative potential of compounds containing, for example, fucoxanthin, fatty acids, peptides, isoflavonoids or terpenes, among others.
AB - This paper demonstrates a sequential partitioning method for isolating bioactive compounds from Chrysochromulina rotalis using a polarity gradient, replacing classic and hazardous solvents with greener alternatives. Seventeen solvents were evaluated based on their Hansen solubility parameters and for having a similar polarity to the solvents they would replace, four of which were selected as substitutes in the classic fractionation process. Considering the fatty acid and carotenoid recovery yields obtained for each of the solvents, it has been proposed to replace hexane (HEX), toluene (TOL), dichloromethane (DCM) and n-butanol (BUT) with cyclohexane, chlorobenzene, isobutyl acetate and isoamyl alcohol, respectively. In addition, cytotoxic activity was observed when the TOL and DCM solvent extracts were tested against tumour cell lines, demonstrating the antiproliferative potential of compounds containing, for example, fucoxanthin, fatty acids, peptides, isoflavonoids or terpenes, among others.
KW - Alternative solvents
KW - Antiproliferative
KW - Chrysochromulina
KW - Fractionation
UR - http://www.scopus.com/inward/record.url?scp=85152567239&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2023.129057
DO - 10.1016/j.biortech.2023.129057
M3 - Artículo
C2 - 37059341
AN - SCOPUS:85152567239
SN - 0960-8524
VL - 379
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 129057
ER -
